Investigations of permeability, diapause, and hatching in the eggs of the mosquito Aedes hexodontus Dyar
Investigations of permeability, diapause, and hatching in the eggs of the mosquito Aedes hexodontus Dyar
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DOI：
10.1139/z58-050
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发表时间：
1958-01-01
期刊：
影响因子：
--
通讯作者：
BECKEL, W. E.
中科院分区：
文献类型：
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作者：
BECKEL, W. E.
描述了在实验室中从现场采集的、用血液喂养的雌性中采集数千个卵子的方法。还概述了一种分离受精卵和未受精卵的方法。产下的蛋是白色的，但 1/2 小时内开始变暗。当卵细胞或早期胚胎被杀死时，颜色并没有发生。观察到六齿伊蚊卵滞育前阶段的两种渗透性变化。第一次发生在绒毛膜从白色变成黑色时。鸡蛋第一次产下时很容易失水和吸水，但随着绒毛膜变黑，它会抵制失水和吸收水分。然而，并没有实现完全防水。随着透明角质层的形成，渗透性发生了进一步的变化。即使在极度干燥的情况下，鸡蛋也几乎不损失任何水分，而且损失的少量水分恢复得非常缓慢。然而，在次氯酸钠中除去绒毛膜后的角质层具有相当的渗透性。绒毛膜和角质层组合的不渗透性必定是由两者之间的结合造成的，并且这种结合必须通过去绒毛膜作用而被破坏。为了研究发生在蚊子胚胎发育晚期的专性滞育，研究了为胚胎提供水、光和低温的影响。当胚胎有水时，滞育不会被打破，室温或较低温度下的光照和黑暗交替也不会破坏滞育。为了测试寒冷的影响，使用1°和-3%的温度来模拟现场温度。人们发现低温终止了滞育，但孵化的卵数量并没有随着暴露在寒冷中的时间的增加而稳定增加。尚未发现任何线索来解释这些结果。研究发现，将腐烂的成年蚊子尸体注入蒸馏水中进行孵化，比单独使用蒸馏水的孵化率更高。孵化刺激的机制尚不清楚。 ||摘要作者：Auth。抽象
Methods for collection in the laboratory of many thousands of eggs from field-collected, blood-fed females are described. Also a way of separating fertile from infertile eggs is outlined. When the eggs are laid they are white but begin to darken within 1/2 hour. The darkening did not occur when the egg cell or early embryo was killed. Two changes in permeability in the prediapause stage of the eggs of Aedes hexodontus were observed. The 1st took place as the chorion changed from white to black. The egg lost and gained water easily when 1st laid but resisted water loss and uptake as the chorion darkened. However, a complete waterproofing did not result. With the formation of the transparent cuticle a further change in permeability occurred. The egg lost hardly any water even when exposed to extreme desiccation and what little water was lost was regained very slowly. However, the cuticle, with the chorion removed in sodium hypochlorite, was quite permeable. The impermeability of the chorion and cuticle combination must result from the bonding between the 2 and this bonding must be disrupted by the dechorionation. To study the obligate diapause that occurs in the late embryogeny of the mosquito egg the effect of making water available to the embryo, of light, and of low temperature was studied. Diapause was not broken when water was available to the embryo, nor did alternate periods of light and dark at room temperature or at lower temperatures disrupt the diapause. To test the influence of cold the temperature of 1[degree]and -3% were used to simulate the temperatures in the field. The low temperatures were found to terminate the diapause but the numbers of eggs hatching did not steadily increase with an increase in the time of exposure to cold. No clue has as yet been discovered to explain these results. A hatching infusion of decaying adult mosquito bodies in distilled water was found to give more hatching than did distilled water alone. The mechanism of the hatching stimulus is not known. || ABSTRACT AUTHORS: Auth. abst