“Blinking” silica nanoparticles for optical super resolution imaging of cancer cells
“Blinking” silica nanoparticles for optical super resolution imaging of cancer cells
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DOI：
10.1039/c7ra08156a
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发表时间：
2017-10
期刊：
影响因子：
3.9
通讯作者：
Jia Li;S. Zong;Zhuyuan Wang;Yiping Cui
中科院分区：
文献类型：
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作者：
Jia Li;S. Zong;Zhuyuan Wang;Yiping Cui
单分子定位显微镜（SMLM）的荧光团必须表现出连续的信号波动（即闪烁）。在这里，我们提出了一种使用Alexa Fluor 647（A647）使二氧化硅纳米颗粒（NPs）闪烁的策略，这是一种广泛用于SMLM的有机染料。我们发现，将A647封装在二氧化硅纳米颗粒内部产生不闪烁的二氧化硅纳米颗粒，而将A647装饰到二氧化硅纳米颗粒的外表面上产生闪烁的二氧化硅纳米颗粒。研究了闪烁二氧化硅纳米粒子的形貌和荧光性质。此外，通过附着癌症特异性抗体，闪烁的二氧化硅纳米颗粒可以特异性地靶向癌细胞。使用共聚焦激光扫描显微镜和SMLM对掺入闪烁二氧化硅NP的细胞进行成像。这种具有光学超分辨成像能力的肿瘤特异性闪烁纳米粒子在深入研究肿瘤细胞与纳米粒子之间的相互作用方面具有很大的潜力。
Fluorophores for single molecule localization microscopy (SMLM) must exhibit continuous signal fluctuations (i.e. blinking). Here, we present a strategy to make silica nanoparticles (NPs) blink using Alexa Fluor 647 (A647), an organic dye widely used in SMLM. We find that encapsulating A647 inside the silica NPs produces non-blinking silica NPs, while decorating A647 onto the outer surface of silica NPs generates blinking silica NPs. The morphology and fluorescence properties of the blinking silica NPs are studied. Besides, by attaching cancer specific antibodies, the blinking silica NPs can specifically target the cancer cells. Cells incorporated with the blinking silica NPs are imaged using confocal laser scanning microscopy and SMLM. With the optical super resolution imaging ability, this kind of cancer specific blinking silica NPs can have a great potential in detailed investigation of the interaction between cancer cells and NPs.