Diet Versus Phylogeny: a Comparison of Gut Microbiota in Captive Colobine Monkey Species
Diet Versus Phylogeny: a Comparison of Gut Microbiota in Captive Colobine Monkey Species
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DOI：
10.1007/s00248-017-1041-8
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发表时间：
2018-02-01
影响因子：
3.6
通讯作者：
Amato, Katherine R.
中科院分区：
文献类型：
--
作者：
Hale, Vanessa L.;Tan, Chia L.;Amato, Katherine R.
饮食和宿主系统发育都塑造了肠道微生物群落，分离出这些变量的影响可能是具有挑战性的。本研究利用高通量测序技术，研究了食性和系统发育对9种东亚钳猴(N=个体)肠道微生物区系的影响。眼镜蛇是一种食叶猴子，在圈养中表现不佳--通常表现出胃肠道(GI)问题。这项研究包括8种亚洲孔雀(Rhinopithecus brelichi、Rhinopithecus roxellana、Rhinopithecus bieti、Pygathrix nemaeus、Nasalis larvatus、Trachypithecus frcoisi、Trachypithecus auratus和Trachypithecus vetulus)和1种非洲Colobine(Colobus Guereza)。猴子被关在五个不同的圈养机构：攀西野生动物救援中心(贵州，中国)，北京动物园，北京动物园繁育中心，新加坡动物园，新加坡动物园灵长类动物保护繁育中心。圈养的饮食在不同的机构之间差别很大，但在一个机构内，所有种类的共生猕猴都得到了几乎相同或相同的饮食。此外，在多个圈养机构中还发现了四种猴子。这使我们能够分析这些圈养的袋鼠的饮食和系统发育的影响。肠道微生物群落按寄主物种聚集较弱，而受饮食聚集较强，总体上，在肠道微生物区系分析中没有反映出丛枝杆菌的系统发育关系。核心微生物区系分析还确定了几个关键的分类群--包括反刍球菌科和乳螺科中的微生物--这项研究中超过90%的猴子都有这些微生物。这些家族中的微生物种类包括许多对胃肠道健康很重要的丁酸盐生产者。这些结果突显了圈养仓鼠饮食的重要性。
Both diet and host phylogeny shape the gut microbial community, and separating out the effects of these variables can be challenging. In this study, high-throughput sequencing was used to evaluate the impact of diet and phylogeny on the gut microbiota of nine colobine monkey species (N = 64 individuals). Colobines are leaf-eating monkeys that fare poorly in captivity-often exhibiting gastrointestinal (GI) problems. This study included eight Asian colobines (Rhinopithecus brelichi, Rhinopithecus roxellana, Rhinopithecus bieti, Pygathrix nemaeus, Nasalis larvatus, Trachypithecus francoisi, Trachypithecus auratus, and Trachypithecus vetulus) and one African colobine (Colobus guereza). Monkeys were housed at five different captive institutes: Panxi Wildlife Rescue Center (Guizhou, China), Beijing Zoo, Beijing Zoo Breeding Center, Singapore Zoo, and Singapore Zoo Primate Conservation Breeding Center. Captive diets varied widely between institutions, but within an institution, all colobine monkey species were fed nearly identical or identical diets. In addition, four monkey species were present at multiple captive institutes. This allowed us to parse the effects of diet and phylogeny in these captive colobines. Gut microbial communities clustered weakly by host species and strongly by diet, and overall, colobine phylogenetic relationships were not reflected in gut microbiota analyses. Core microbiota analyses also identified several key taxa-including microbes within the Ruminococcaceae and Lachnospiraceae families-that were shared by over 90% of the monkeys in this study. Microbial species within these families include many butyrate producers that are important for GI health. These results highlight the importance of diet in captive colobines.