Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
复制标题
DOI：
10.1038/31159
复制
发表时间：
1998-06-11
期刊：
影响因子：
64.8
通讯作者：
Barrell, BG
中科院分区：
文献类型：
--
作者：
Cole, ST;Brosch, R;Barrell, BG
无数人死于结核病，这是一种由结核杆菌引起的慢性传染病。已确定并分析了最具特征的结核分枝杆菌H37Rv菌株的全基因组序列，以提高我们对这种生长缓慢的病原体的生物学认识，并有助于构思新的预防和治疗干预措施。基因组包含4,411,529个碱基对，包含约4,000个基因，并且具有非常高的鸟嘌呤+胞嘧啶含量，这反映在蛋白质的偏氨基酸含量上。结核分枝杆菌与其他细菌的根本不同之处在于，它的很大一部分编码能力专门用于生产参与脂肪生成和脂肪分解的酶，以及两个新的富含甘氨酸的蛋白质家族，这些蛋白质具有重复的结构，可能代表抗原变异的来源。
Countless millions of people have died from tuberculosis, a chronic infectious disease caused by the tubercle bacillus. The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve our understanding of the biology of this slow-growing pathogen and to help the conception of new prophylactic and therapeutic interventions. The genome comprises 4,411,529 base pairs, contains around 4,000 genes, and has a very high guanine + cytosine content that is reflected in the biased amino-acid content of the proteins. M. tuberculosis differs radically from other bacteria in that a very large portion of its coding capacity is devoted to the production of enzymes involved in lipogenesis and lipolysis, and to two new families of glycine-rich proteins with a repetitive structure that may represent a source of antigenic variation.