Colonic epithelial cell proliferation in hereditary non-polyposis colorectal cancer
Colonic epithelial cell proliferation in hereditary non-polyposis colorectal cancer
复制标题
遗传性非息肉病性结直肠癌中结肠上皮细胞增殖
作者：
S. E. Green;P. Chapman;J. Burn;A. Burt;M. Bennett;D. Appleton;J. Varma;J. Mathers
背景-尽管最近发现了高达90%的遗传性非息肉病性结直肠癌(HNPCC)病例的四个基因，但仍有一些家庭无法进行预测性检测。因此，表型生物标志物将是有用的。据报道，结肠隐窝内的增殖室上移是癌前病变最早的改变之一。目的：评估隐窝细胞增殖作为HNPCC表型生物标志物的作用。患者-35名HNPCC基因携带率为50%的患者(其中21人随后接受了预测性检测，因此基因携带者状况已知)和18名对照组。方法：使用两种不同的技术在结肠的五个部位测量隐窝细胞的增殖。用单抗MIB1测定标记指数，用显微解剖和压榨技术测定全隐窝有丝分裂指数。此外，还评估了隐窝内增殖细胞的分布情况。结果：在任何部位，研究组和对照组在总标记指数或每个隐窝的平均有丝分裂数上没有显著差异，在隐窝内增殖细胞的分布也没有显著差异。对已知基因携带者的21例患者分别进行分析，HNPCC基因携带者与非基因携带者的增殖指数差异无统计学意义。结论：隐窝细胞增殖不是HNPCC基因携带的鉴别标志。
Background—Despite the recent discovery of four genes responsible for up to 90% of all cases of hereditary non-polyposis colorectal cancer (HNPCC), there will still be families in whom predictive testing is not possible. A phenotypic biomarker would therefore be useful. An upwards shift of the proliferative compartment in colonic crypts is reported to be one of the earliest changes in premalignant mucosa. Aims—To assess the role of crypt cell proliferation as a phenotypic biomarker in HNPCC. Patients—Thirty five patients at 50% risk of carrying the HNPCC gene (21 of whom subsequently underwent predictive testing and hence gene carrier status was known) and 18 controls. Methods—Crypt cell proliferation was measured at five sites in the colon using two different techniques. Labelling index was determined using the monoclonal antibody MIB1 and whole crypt mitotic index was measured using the microdissection and crypt squash technique. The distribution of proliferating cells within the crypts was also assessed. Results—There were no significant differences in the total labelling index or mean number of mitoses per crypt, nor in the distribution of proliferating cells within the crypt, between the study and control groups at any site. When the 21 patients in whom gene carrier status was known were analysed separately there were no significant differences in the measured indices of proliferation between the HNPCC gene carriers and non-gene carriers. Conclusion—Crypt cell proliferation is not a discriminative marker of gene carriage in HNPCC.
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影响因子：
64.5
作者：
FISHEL, R;LESCOE, MK;KOLODNER, R
通讯作者：
KOLODNER, R
结肠上皮增殖作为遗传性非息肉病性结肠癌美洲原住民家庭生物标志物的后续研究。
DOI：
10.1093/jnci/83.13.951
发表时间：
1991
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Lynch,PM;Wargovich,MJ;Lynch,HT;Palmer,C;Lanspa,S;Drouhard,T;Lynch,J
通讯作者：
Lynch,J
验证增殖指数作为替代终点生物标志物。
DOI：
--
发表时间：
1994
期刊：
Journal of cellular biochemistry. Supplement
影响因子：
--
作者：
Alberts,DS;Einspahr,J;Aickin,M;Hixson,L;Earnest,D;Roe,D;Powell,M
通讯作者：
Powell,M
影响因子：
4.4
作者：
KOLODNER, RD;HALL, NR;BISHOP, DT
通讯作者：
BISHOP, DT