INTERSPECIES RELATIONSHIPS IN THE PARAMECIUM-AURELIA COMPLEX - ACID-PHOSPHATASE VARIATION
INTERSPECIES RELATIONSHIPS IN THE PARAMECIUM-AURELIA COMPLEX - ACID-PHOSPHATASE VARIATION
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DOI：
10.1111/j.1550-7408.1983.tb01048.x
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
RUSHFORD, CL
中科院分区：
文献类型：
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作者：
ALLEN, SL;ADAMS, J;RUSHFORD, CL
14种中的13种经洗涤剂处理后的提取物电泳后，凝胶中出现了多达5个酸性磷酸酶活性区带。奥雷利亚复合体的一部分。所有物种的总体模式有点相似：个别区域的强度和流动性差异允许将这些兄弟物种分为8组。所有14种属可以使用酶电泳程序进行鉴定，尽管其中2个比通常情况下更相似。错误分类的问题进行了讨论的性质和频率的变体。通过明智地选择用于筛选新股票的酶，这些问题可以避免。使用11种酶更新物种关系。从遗传距离矩阵构建的树状图显示了4个物种集群：（i）双尾拟单胞菌、三尾拟单胞菌;（ii）原尾拟单胞菌、五尾拟单胞菌、六尾拟单胞菌、新尾拟单胞菌;（iii）七尾拟单胞菌、十一尾拟单胞菌、十三尾拟单胞菌、四尾拟单胞菌;和（iv）四尾拟单胞菌、八尾拟单胞菌、十尾拟单胞菌、十二尾拟单胞菌。物种之间的距离很大，在果蝇之间的差异。种的特征是非常缺乏地理分化和巨大的形态相似性，这与分子分化形成强烈对比。
Up to 5 zones of acid phosphatase activity appear in gels after electrophoresis of detergent-treated extracts from 13 of the 14 spp. of the P. aurelia complex. The overall pattern is somewhat similar for all species: differences in intensity and mobility of individual zones permit the grouping of these sibling species into 8 groups. All 14 spp. can be identified using the procedure of enzyme electrophoresis, although 2 of them are more similar than is usually the case. Problems of misclassification are discussed in terms of the nature and frequency of variants. With the judicious choice of enzymes used to screen new stocks, these problems can be circumvented. Species relationships are updated using 11 enzymes. A dendrogram constructed from the matrix of genetic distances shows 4 clusters of species: (i) P. biaurelia, P. triaurelia; (ii) P. primaurelia, P. pentaurelia, P. sexaurelia, P. novaurelia; (iii) P. septaurelia, P. undecaurelia, P. tredecaurelia, P. quadecaurelia; and (iv) P. tetraurelia, P. octaurelia, P. decaurelia, P. dodecaurelia. Distances between the species are large, on the order of the differences between Drosophila spp. The species are characterized by an extraordinary lack of geographical differentiation and great morphological similarity, which contrasts strongly with the molecular differentiation.