SUBSETS OF MIDBRAIN DOPAMINERGIC-NEURONS IN MONKEYS ARE DISTINGUISHED BY DIFFERENT LEVELS OF MESSENGER-RNA FOR THE DOPAMINE TRANSPORTER - COMPARISON WITH THE MESSENGER-RNA FOR THE D-2 RECEPTOR, TYROSINE-HYDROXYLASE AND CALBINDIN IMMUNOREACTIVITY
SUBSETS OF MIDBRAIN DOPAMINERGIC-NEURONS IN MONKEYS ARE DISTINGUISHED BY DIFFERENT LEVELS OF MESSENGER-RNA FOR THE DOPAMINE TRANSPORTER - COMPARISON WITH THE MESSENGER-RNA FOR THE D-2 RECEPTOR, TYROSINE-HYDROXYLASE AND CALBINDIN IMMUNOREACTIVITY
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DOI：
10.1002/cne.903620308
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发表时间：
1995-11-20
影响因子：
2.5
通讯作者：
LU, W
中科院分区：
文献类型：
--
作者：
HABER, SN;RYOO, H;LU, W
中脑多巴胺系统根据化学特性和连接性可分为两组细胞。背侧层神经元（包括致密部背侧部分和腹侧被盖区）钙结合蛋白呈阳性，并投射到伏隔核的壳部。腹侧层神经元钙结合蛋白呈阴性，并投射到感觉运动纹状体。本研究利用原位杂交技术检测了猴子中脑多巴胺转运体分子（DAT）和D - 2受体的mRNA分布。将其分布模式与酪氨酸羟化酶和钙结合蛋白免疫组织化学的分布模式进行了比较。结果显示，在腹侧层钙结合蛋白阴性的神经元中发现DAT和D - 2受体mRNA的高水平杂交，而在背侧钙结合蛋白阳性层中发现相对较低水平。在背侧层内，黑质致密部背侧部分两种信息的含量最少。这些结果表明，在猴子中，腹侧被盖区和致密部背侧部分形成了一个多巴胺神经元的背侧连续体，其DAT和D - 2受体的mRNA表达水平低于腹侧层。DAT已被证明与N - 甲基 - 4 - 苯基 - 1,2,3,6 - 四氢吡啶（MPTP）的选择性神经毒性有关。DAT mRNA和钙结合蛋白的不同水平可能解释了MPTP神经毒性的不同影响。（C）1995威利 - 利斯公司
The midbrain dopamine system can be divided into two groups of cells based on chemical characteristics and connectivity. The dorsal tier neurons, which include the dorsal pars compacta and the ventral tegmental area, are calbindin-positive, and project to the shell of the nucleus accumbens. The ventral tier neurons are calbindin-negative and project to the sensorimotor striatum. This study examined the distribution of the mRNAs for the dopamine transporter molecule (DAT) and the D-2 receptor in the midbrain of monkeys by using in situ hybridization. The distribution patterns were compared to that of tyrosine hydroxylase and calbindin immunohistochemistry. The results show that high levels of hybridization for DAT and the D-2 receptor mRNA are found in the ventral tier, calbindin-negative neurons and relatively low levels are found in the dorsal, calbindin-positive tier. Within the dorsal tier, the dorsal substantia nigra pars compacta has the least amount of both messages. These results show that in monkeys, the ventral tegmental area and the dorsal pars compacts form a dorsal continuum of dopamine neurons which express lower levels of mRNA for DAT and D-2 receptor than the ventral tier. DAT has been shown to be involved in the selective neurotoxicity of N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Different levels of DAT mRNA and calbindin may explain the differential effects of MPTP neurotoxicity. (C) 1995 Wiley-Liss, Inc.