Corrigendum to "Dynamic behavior of cell-free mitochondrial DNA in human saliva" [Psychoneuroendocrinology 143 (2022) 105852].
Corrigendum to "Dynamic behavior of cell-free mitochondrial DNA in human saliva" [Psychoneuroendocrinology 143 (2022) 105852].
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“人类唾液中无细胞线粒体 DNA 的动态行为”的勘误表 [Psychoneuroendocrinology 143 (2022) 105852]。
DOI：
10.1016/j.psyneuen.2022.105884
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发表时间：
2022
影响因子：
3.7
通讯作者：
Picard,Martin
中科院分区：
文献类型：
--
作者：
Trumpff,Caroline;Rausser,Shannon;Haahr,Rachel;Karan,KalpitaR;Gouspillou,Gilles;Puterman,Eli;Kirschbaum,Clemens;Picard,Martin
报告了 Caroline Trumpff、Shannon Rausser、Rachel Haahr、Kalpita R. Karan、Gilles Gouspillou、Eli Puterman、Clemens Kirschbaum 和 Martin Picard 的“人类唾液中无细胞线粒体 DNA 的动态行为”中的错误（心理神经内分泌学，2022 年 [9 月]，第 143 卷，1-12）。第 9 页的方法部分出现印刷错误，其中试剂浓度输入错误。裂解缓冲液中 Tris HCL 的浓度应为 38 mM，而不是 100 mM。提供了更正的句子。（以下原始文章摘要出现在记录 2022-87832-001 中）。线粒体含有自己的基因组，可以以无细胞线粒体 DNA (cf-mtDNA) 的形式释放到血液和脑脊液等多种生物流体中。在临床研究中，血液 cf-mtDNA 可以预测死亡率，而较高的 cf-mtDNA 水平与精神和身体压力有关。然而，cf-mtDNA 的动态尚未确定，并且是否可以像唾液中的其他神经内分泌标记物一样进行非侵入性测量尚未得到检验。在这里，我们报告了人类唾液中的 cf-mtDNA，并建立了其在数周内的自然人体内动态行为。在一小群健康成年人的原理验证中，我们首先开发了一种无需 DNA 分离即可快速量化唾液 cf-mtDNA 的方法，并证明了唾液 cf-mtDNA 的存在。然后，我们采用这种方法对两名健康男性进行密集的重复测量分析，在连续 53-60 天的 4 个每日时间点进行研究（每个时间点 n= 212-220 个观察值），并平行测量类固醇激素、自我报告的日常情绪和健康相关行为。唾液cf-mtDNA表现出强烈的觉醒反应，在觉醒后30-45分钟达到两个数量级，每天都有变化，并且与皮质醇觉醒反应适度相关。在探索性分析中，没有发现与自我报告的日常情绪/健康相关行为之间存在一致的关联，尽管这需要在未来的研究中进一步检查。 cf-mtDNA 的动态变化与唾液白细胞介素 6 (IL-6) 呈负相关，与唾液 cf-mtDNA 的促炎作用不一致。唾液 cf-mtDNA 的高度动态行为为非侵入性研究打开了大门，研究 mtDNA 信号传导与人类健康的相关性。（PsycInfo 数据库记录 (c) 2022 APA，保留所有权利）
Reports an error in" Dynamic behavior of cell-free mitochondrial DNA in human saliva" by Caroline Trumpff, Shannon Rausser, Rachel Haahr, Kalpita R. Karan, Gilles Gouspillou, Eli Puterman, Clemens Kirschbaum and Martin Picard (Psychoneuroendocrinology, 2022 [Sep], Vol 143, 1-12). A typographical error was introduced in the methods section on page 9, where the concentration of a reagent was mistyped. The concentration of Tris HCL in the lysis buffer should be 38 mM rather than 100 mM. The corrected sentence is provided.(The following abstract of the original article appeared in record 2022-87832-001). Mitochondria contain their own genome that can be released in multiple biofluids such as blood and cerebrospinal fluid, as cell-free mitochondrial DNA (cf-mtDNA). In clinical studies, blood cf-mtDNA predicts mortality and higher cf-mtDNA levels are associated with mental and physical stress. However, the dynamics of cf-mtDNA has not been defined, and whether it can be measured non-invasively like other neuroendocrine markers in saliva has not been examined. Here we report cf-mtDNA in human saliva and establish its natural within-person dynamic behavior across multiple weeks. In a small proof-of-principle cohort of healthy adults, we first develop an approach to rapidly quantify salivary cf-mtDNA without DNA isolation, and demonstrate the existence of salivary cf-mtDNA. We then deploy this approach to perform an intensive repeated-measures analysis of two healthy men studied at 4 daily timepoints over 53–60 consecutive days (n= 212–220 observations each) with parallel measures of steroid hormones, self-reported daily mood, and health-related behaviors. Salivary cf-mtDNA exhibited a robust awakening response reaching up to two orders of magnitude 30–45 min after awakening, varied from day-to-day, and moderately correlated with the cortisol awakening response. In exploratory analyses, no consistent association with self-reported daily mood/health-related behaviors were found, although this requires further examination in future studies. Dynamic variation in cf-mtDNA was inversely related with salivary interleukin 6 (IL-6), inconsistent with a pro-inflammatory effect of salivary cf-mtDNA. The highly dynamic behavior of salivary cf-mtDNA opens the door to non-invasive studies examining the relevance of mtDNA signaling in relation to human health.(PsycInfo Database Record (c) 2022 APA, all rights reserved)