Remodeling of the leg sensory system during metamorphosis of the hawkmoth, Manduca sexta
Remodeling of the leg sensory system during metamorphosis of the hawkmoth, Manduca sexta
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DOI：
10.1002/(sici)1096-9861(20000403)419:2
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发表时间：
2000-04
影响因子：
2.5
通讯作者：
C. Consoulas
中科院分区：
文献类型：
--
作者：
C. Consoulas
成年的Manduca sexta的腿是由一系列不同的感觉器官和相关神经元提供的（Kent and Griffin [1990] Cell Tissue Res. 259:209-223），与幼虫的腿不同。在本研究中，神经示踪技术[生物细胞素，1,1 ' -二十八烷基- 3,3,3 ',3 ' -四甲基-吲哚碳青高氯酸盐（DiI）]，出生日期标记（5 -溴脱氧尿苷），共聚焦显微镜和电生理学的组合被用来描述前胸腿感觉系统的重塑。四条主要的感觉分支携带着幼虫腿上所有感觉神经元的轴突。在变态开始时，想象的腿表皮在幼虫角质层下发育并包围感觉神经元，从而将它们与目标器官分开。大多数幼虫神经元在幼虫到蛹的转变过程中退化，并被新的成体感觉神经元所取代，这些神经元在蛹中出生并分化。在幼体腿中提供毛感器的6个感觉神经元，以及13个股骨和胫骨腱束器官神经元，持续存在于发育中的成体腿中，具有类似的功能。在蛹期早期，尽管没有目标感觉结构，但这些神经元的电活动可以被记录下来。在成体感觉系统的分化过程中，新生-成体感觉神经元的轴突与持久神经元的轴突接触并形成束状。因此，成虫腿的五个主要感觉分支是建立在先前存在的幼虫感觉轨迹上的。然而，两个感觉分支是由特定成年感觉神经元的轴突重新建立的。[j] .中华神经医学杂志，2001,19(4):357 - 357。©2000 Wiley‐Liss, Inc。
The adult legs of the hawkmoth Manduca sexta are supplied by a diverse array of sensory organs and associated neurons (Kent and Griffin [ 1990 ] Cell Tissue Res. 259:209–223) that differ from those in the larval legs. In the present study, a combination of nerve‐tracing techniques [biocytin, 1,1`‐dioctadecyl‐3,3,3`,3`‐tetramethyl‐indocarbocyanine perchlorate (DiI)], birth date labeling (5‐bromodeoxyuridine), confocal microscopy, and electrophysiology were used to describe the remodeling of the prothoracic leg sensory system. Four primary sensory branches carry the axons of all of the sensory neurons in the larval leg. At the onset of metamorphosis, the imaginal leg epidermis develops underneath the larval cuticle and encircles the sensory neurons, thus separating them from their target‐organs. Most of the larval neurons degenerate during the larval‐to‐pupal transition and are replaced by new‐adult sensory neurons that are born and differentiate in the pupa. Six sensory neurons that supply hair sensilla in the larval leg, together with 13 femoral and tibial chordotonal organ neurons, persist into the developing adult leg to serve similar functions. Early in the pupal stage, electrical activity can be recorded from these neurons despite the absence of target sensory structures. During the differentiation of the adult sensory system, the axons of the new‐adult sensory neurons contact and fasciculate with the axons of the persistent neurons. Thus, five of the primary sensory branches of the adult leg are built on the preexisting larval sensory trajectories. Two sensory branches, however, are established de novo by the axons of specific adult sensory neurons. J. Comp. Neurol. 419:154–174, 2000. © 2000 Wiley‐Liss, Inc.