INDUCTION AND EXPRESSION OF LONG-TERM AND SHORT-TERM NEUROSECRETORY POTENTIATION IN A NEURAL CELL-LINE
INDUCTION AND EXPRESSION OF LONG-TERM AND SHORT-TERM NEUROSECRETORY POTENTIATION IN A NEURAL CELL-LINE
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DOI：
10.1016/0896-6273(90)90347-i
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发表时间：
1990-12-01
期刊：
影响因子：
16.2
通讯作者：
KOSHLAND, DE
中科院分区：
文献类型：
--
作者：
MORIMOTO, BH;KOSHLAND, DE
在神经细胞系中，添加血清素会增强响应去极化刺激的兴奋性氨基酸的分泌。 这种增强的持续时间取决于刺激的强度。 强刺激诱导的持续分泌增强需要激活血清素和 NMDA 受体。 在血清素呈递过程中抑制 NMDA 受体可以阻止增强作用的诱导。 增强的时间特征与 cAMP 水平的升高相关。抑制 NMDA 受体时暴露血清素会导致 cAMP 水平短暂升高，而 NMDA 和血清素共激活会导致 cAMP 持续升高。 因此，有可能在单个细胞中瞬时或持续地获得分泌增强。 该系统中增强反应的时间与用作短期和长期记忆模型的类似系统具有相同的幅度。
In a neural cell line, the secretion of excitatory amino acids in response to a depolarizing stimulus is potentiated by the addition of serotonin. The duration of this potentiation is dependent on the strength of the stimulus. Persistent secretory potentiation induced by a strong stimulus requires the activation of both serotonin and NMDA receptors. Inhibiting the NMDA receptor during serotonin presentation prevented the induction of potentiation. The temporal characteristic of the potentiation is correlated with the elevation of cAMP levels. Serotonin exposure while inhibiting NMDA receptors results in a transient elevation of cAMP levels, whereas coactivation with NMDA and serotonin results in a persistent elevation of cAMP. Thus, it is possible to obtain potentiation of secretion in a single cell either transiently or persistently. The timing of potentiated responses in this system is of the same magnitude as that in similar systems used as models for short-term and long-term memory.