Specific globin mRNAs in human erythroleukemia (K562) cells.
Specific globin mRNAs in human erythroleukemia (K562) cells.
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DOI：
10.1182/blood.v63.1.195.195
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发表时间：
1984
期刊：
影响因子：
20.3
通讯作者：
C. Miller;K. Young;D. Duménil;B. Alter;J. Schofield;A. Bank
中科院分区：
文献类型：
--
作者：
C. Miller;K. Young;D. Duménil;B. Alter;J. Schofield;A. Bank
在没有氯化血红素和存在氯化高铁血红素的情况下，几种K562细胞系中特异性珠蛋白mRNA的积聚是定量的。使用特定克隆的DNA探针，发现在20微米的氯化血红素存在下，Zeta、α、epsilon和Gamma的mRNAs数量增加了2-3倍。在所有的品系中都没有检测到β-或β-珠蛋白的mRNAs。在一个品系Bos中，epsilon-珠蛋白mRNA显著减少，随着氯化血红素的增加而增加，尽管水平仍然比其他品系低得多。牛的表珠蛋白基因转录减少是由于表珠蛋白基因转录减少所致。
Specific globin mRNA accumulation was quantitated in several lines of K562 cells in the absence and the presence of hemin. Using specific cloned DNA probes, the amounts of zeta, alpha, epsilon and gamma mRNAs were shown to be increased 2-3-fold in the presence of 20 microM hemin. No delta- or beta-globin mRNAs were detectable in any of the lines. In one line, Bos, there was a marked decrease in epsilon-globin mRNA, which increased with hemin, although still to much lower levels than in the other lines. The decreased epsilon-globin mRNA accumulation in Bos is shown to be due to decreased epsilon-globin gene transcription.