Development of virus-like particles for diagnostic and prophylactic biomedical applications.
Development of virus-like particles for diagnostic and prophylactic biomedical applications.
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DOI：
10.1002/wnan.1336
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Douglas T
中科院分区：
文献类型：
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作者：
Schwarz B;Douglas T
作为有序的纳米级结构，病毒和病毒样颗粒（VLP）仍然无法通过合成策略产生均匀和对称的纳米颗粒。VLP保持或模拟致病病毒的对称性，为促进免疫系统的识别、摄取和处理提供了一个现成的平台。对病毒如何与免疫系统相互作用的新认识提供了一种精确设计用于生物医学用途的纳米颗粒的方法，无论是颗粒的结构还是它们刺激免疫系统的能力。在这里，我们讨论了最近的进展，我们的小组对两个平行和互补的应用VLP，主要来自植物，噬菌体和非病毒来源，在生物医学：诊断成像和合理的疫苗设计。首先，我们讨论了增加钆磁共振成像（MRI）造影剂的VLP有效载荷以及控制单个含钆分子的特性以提高疗效的进展。为了更好地了解VLP构建体的体内潜力，我们然后讨论了蛋白笼和免疫系统的接口，从非特异性先天免疫系统刺激开始，并继续使用非致病性VLP作为特异性抗原呈递和控制免疫应答的支架。
As ordered nanoscale architectures, viruses and virus-like particles (VLPs) remain unsurpassed by synthetic strategies to produce uniform and symmetric nanoparticles. Maintaining or mimicking the symmetry of pathogenic viruses, VLPs offer a ready platform for facilitating recognition, uptake, and processing by the immune system. An emerging understanding of how viruses interact with the immune system offers a means of precisely designing nanoparticles for biomedical use, both with respect to the structure of the particle as well as their ability to stimulate the immune system. Here we discuss recent advances by our group toward two parallel and complementary applications of VLPs, derived primarily from plants, bacteriophage, and nonviral sources, in biomedicine: diagnostic imaging and rational vaccine design. First we discuss advances in increasing VLP payloads of gadolinium magnetic resonance imaging (MRI) contrast agent as well as controlling the characteristics of individual gadolinium containing molecules to increase efficacy. In order to better understand the in vivo potential of VLP constructs, we then discuss the interface of protein-cages and the immune system beginning with the nonspecific innate immune system stimulation and continuing into the use of non-pathogenic VLPs as scaffolds for specific antigen presentation and control of the immune response.