Mapping of In Vivo RNA-Binding Sites by Ultraviolet (UV)-Cross-Linking Immunoprecipitation (CLIP).
Mapping of In Vivo RNA-Binding Sites by Ultraviolet (UV)-Cross-Linking Immunoprecipitation (CLIP).
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DOI：
10.1101/pdb.top097931
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发表时间：
2018-12
影响因子：
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通讯作者：
J. Darnell;Aldo Mele;K. Hung;R. Darnell
中科院分区：
文献类型：
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作者：
J. Darnell;Aldo Mele;K. Hung;R. Darnell
RNA“CLIP”（交联免疫沉淀）是一种将RNA-蛋白质复合物共价交联并纯化以及RNA测序的方法，作为开发直接的、功能性RNA-蛋白质相互作用位点的全基因组图谱的有力手段而引起了人们的关注。这些图谱已被用于识别调控点，它们有望了解正常细胞功能中RNA调控的动态及其在疾病中的失调。
RNA "CLIP" (cross-linking immunoprecipitation), the method by which RNA-protein complexes are covalently cross-linked and purified and the RNA sequenced, has attracted attention as a powerful means of developing genome-wide maps of direct, functional RNA-protein interaction sites. These maps have been used to identify points of regulation, and they hold promise for understanding the dynamics of RNA regulation in normal cell function and its dysregulation in disease.