Coherent Raman scattering microscopy: capable solution in search of a larger audience.
Coherent Raman scattering microscopy: capable solution in search of a larger audience.
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DOI：
10.1117/1.jbo.26.6.060601
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发表时间：
2021-06
影响因子：
3.5
通讯作者：
Potma EO
中科院分区：
文献类型：
--
作者：
Prince RC;Potma EO
重要性：相干拉曼散射（CRS）显微镜是一种光学成像技术，其功能可以使广泛的生物医学研究受益。目的：我们反思了该技术的诞生，快速崛起和不可避免的成长痛苦，并回顾了近四十年的发展，以研究CRS成像方法在未来十年的发展方向。方法：我们提供了一个简短的CRS显微镜的历史帐户，随后讨论的挑战，传播技术，以更大的观众。然后，我们强调最近的进展，扩大CRS显微镜的能力，并评估其目前的吸引力作为一个实用的成像工具。结果：拉曼标记技术的新发展提高了CRS技术的特异性和灵敏度。此外，技术进步导致CRS显微镜可以在实际采集时间捕获高光谱数据立方体。这些改进拓宽了该技术的应用空间。结论：自成立以来，CRS显微镜的技术性能已经有了很大的提高，但这些进步还没有转化为一个强大的核心爱好者之外的大量用户群。尽管如此，新的发展已经准备好将这项技术的独特功能转移到更多用户手中。
Significance: Coherent Raman scattering (CRS) microscopy is an optical imaging technique with capabilities that could benefit a broad range of biomedical research studies. Aim: We reflect on the birth, rapid rise, and inescapable growing pains of the technique and look back on nearly four decades of developments to examine where the CRS imaging approach might be headed in the next decade to come. Approach: We provide a brief historical account of CRS microscopy, followed by a discussion of the challenges to disseminate the technique to a larger audience. We then highlight recent progress in expanding the capabilities of the CRS microscope and assess its current appeal as a practical imaging tool. Results: New developments in Raman tagging have improved the specificity and sensitivity of the CRS technique. In addition, technical advances have led to CRS microscopes that can capture hyperspectral data cubes at practical acquisition times. These improvements have broadened the application space of the technique. Conclusion: The technical performance of the CRS microscope has improved dramatically since its inception, but these advances have not yet translated into a substantial user base beyond a strong core of enthusiasts. Nonetheless, new developments are poised to move the unique capabilities of the technique into the hands of more users.