Influence of aging on the quantity and quality of human cardiac stem cells.
Influence of aging on the quantity and quality of human cardiac stem cells.
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DOI：
10.1038/srep22781
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发表时间：
2016-03-07
影响因子：
4.6
通讯作者：
Hamano K
中科院分区：
文献类型：
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作者：
Nakamura T;Hosoyama T;Kawamura D;Takeuchi Y;Tanaka Y;Samura M;Ueno K;Nishimoto A;Kurazumi H;Suzuki R;Ito H;Sakata K;Mikamo A;Li TS;Hamano K
高龄会影响各种组织特异性干细胞并降低其再生能力。因此，我们研究了衰老是否影响心脏干细胞的数量和质量，使用的细胞来自26名不同年龄的患者（从2岁到83岁）。我们收集了新鲜的右心房，并培养了心球源性细胞（cdc），这是一种心脏干细胞。然后我们研究了CDCs的生长速率、衰老、DNA损伤和生长因子的产生。所有样品均产生足够数量的CDCs用于实验，细胞生长速率与年龄无明显相关性。衰老相关的b-半乳糖苷酶和DNA损伤标志物gH2AX的表达在老年患者（≥65岁）的cdc中显示出略高的趋势。VEGF、HGF、IGF-1、SDF-1和TGF-b的表达在不同样本中存在差异，且这些有益因子的表达不随年龄的增长而降低。体外血管生成试验也表明，即使在老年患者中，cdc的血管生成能力也没有受损。我们的数据表明，年龄对cdc数量和质量的影响是相当有限的。这些发现对老年患者自体干细胞移植具有重要的临床意义。
Advanced age affects various tissue-specific stem cells and decreases their regenerative ability. We therefore examined whether aging affected the quantity and quality of cardiac stem cells using cells obtained from 26 patients of various ages (from 2 to 83 years old). We collected fresh right atria and cultured cardiosphere-derived cells (CDCs), which are a type of cardiac stem cell. Then we investigated growth rate, senescence, DNA damage, and the growth factor production of CDCs. All samples yielded a sufficient number of CDCs for experiments and the cellular growth rate was not obviously associated with age. The expression of senescence-associated b-galactosidase and the DNA damage marker, gH2AX, showed a slightly higher trend in CDCs from older patients (≥65 years). The expression of VEGF, HGF, IGF-1, SDF-1, and TGF-b varied among samples, and the expression of these beneficial factors did not decrease with age. An in vitro angiogenesis assay also showed that the angiogenic potency of CDCs was not impaired, even in those from older patients. Our data suggest that the impact of age on the quantity and quality of CDCs is quite limited. These findings have important clinical implications for autologous stem cell transplantation in elderly patients.