Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography.
Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography.
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DOI：
10.1016/j.neuroimage.2013.07.020
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发表时间：
2014-01-15
期刊：
影响因子：
5.7
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Lin ZJ;Ren M;Li L;Liu Y;Su J;Yang SH;Liu H
漫射光学断层扫描（DOT）已被几个小组用于评估人类和动物脑缺血的脑血流动力学。在本研究中，我们将DOT与吲哚青绿（ICG）跟踪方法结合，在两个大脑中动脉闭塞（MCAO）大鼠模型中获得脑血流动力学和血流指数（BFI）的交错图像。为了获得高空间分辨率的体积图像，我们首先将深度补偿算法（DCA）与基于体积网格的大鼠头部模型相结合，在大鼠脑图谱上生成三维DOT。然后，对栓塞性MCAO模型进行溶栓治疗和不溶栓治疗时，采用交错策略收集两种大鼠模型的脑血流动力学和BFI的实验DOT数据。采用综合大鼠atlas引导DOT方法对获得的动物数据进行进一步分析，形成脑血流动力学和BFI的随时间变化的3D图像。特别是，我们能够显示并确定应用于栓塞性缺血模型的溶栓治疗的治疗结果。本文证明，体积DOT能够提供小动物缺血性脑卒中期间和之后的高质量、交错的脑血流动力学和血流灌注图像，具有良好的三维可视化和量化能力。
Diffuse optical tomography (DOT) has been used by several groups to assess cerebral hemodynamics of cerebral ischemia in humans and animals. In this study, we combined DOT with an indocyanine green (ICG)-tracking method to achieve interleaved images of cerebral hemodynamics and blood flow index (BFI) using two middle cerebral artery occlusion (MCAO) rat models. To achieve volumetric images with high-spatial resolution, we first integrated a depth compensation algorithm (DCA) with a volumetric mesh-based rat head model to generate three-dimensional (3D) DOT on a rat brain atlas. Then, the experimental DOT data from two rat models were collected using interleaved strategy for cerebral hemodynamics and BFI during and after ischemic stroke, with and without a thrombolytic therapy for the embolic MCAO model. The acquired animal data were further analyzed using the integrated rat-atlas-guided DOT method to form time-evolving 3D images of both cerebral hemodynamics and BFI. In particular, we were able to show and identify therapeutic outcomes of a thrombolytic treatment applied to the embolism-induced ischemic model. This paper demonstrates that volumetric DOT is capable of providing high-quality, interleaved images of cerebral hemodynamics and blood perfusion in small animals during and after ischemic stroke, with excellent 3D visualization and quantifications.