Oxygen transport by erythrocyte/hemoglobin solution mixtures in an in vitro capillary as a model of hemoglobin-based oxygen carrier performance
Oxygen transport by erythrocyte/hemoglobin solution mixtures in an in vitro capillary as a model of hemoglobin-based oxygen carrier performance
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DOI：
10.1006/mvre.1997.2055
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发表时间：
1998-01-01
影响因子：
3.1
通讯作者：
Hellums, JD
中科院分区：
文献类型：
--
作者：
Page, TC;Light, WR;Hellums, JD
作为基于血红蛋白的氧载体(HBOC)性能的模型，研究了在微血管中流动的红细胞/细胞外血红蛋白混合物的氧传输行为。利用直径24微米的体外实验毛细管模型，对血红蛋白溶液、红细胞悬浮液和红细胞/血红蛋白溶液混合物的氧通量进行了详细的测量。该实验装置包括与双波长显微分光光度计耦合的计算机数据采集和控制。该装置之前已经通过实验测量与预测的红细胞悬浮液或血红蛋白溶液的氧气传输数学模型的良好一致性而得到验证。实验方法被扩展到测量红细胞/血红蛋白溶液中的氧传输。血红蛋白溶液由纯化的或乙醛聚合的牛血红蛋白组成。通过改变细胞外和细胞内的血红蛋白比例，同时保持总的血红蛋白浓度恒定，产生剂量-反应曲线图。还对未混合的红细胞悬浮液和血红蛋白溶液进行了测量，以产生用于比较的限制情况。实验结果的直接比较表明，这两种类型的血红蛋白溶液在摄取和释放氧气方面都比红细胞悬浮液有效得多。胞外血红蛋白浓度的增加提高了摄取和释放的氧气传输效率，即使在总血红蛋白浓度保持不变的情况下，当细胞外仅占总血红蛋白的10%时，提高的效率约为纯血红蛋白溶液的一半。当细胞外血红蛋白占总血红蛋白的50%时，提高的效率几乎与纯血红蛋白溶液相当。(C)1998年学术出版社。
Oxygen transport behavior of erythrocyte/extracellular hemoglobin mixtures flowing in microvessels was studied as a model of hemoglobin-based oxygen carrier (HBOC) performance. An experimental in vitro 24-mu m-diameter capillary model was used to provide detailed oxygen flux measurements for hemoglobin solutions, erythrocyte suspensions, and erythrocyte/hemoglobin solution mixtures. The experimental apparatus includes computerized data acquisition and control coupled to a dual wavelength microspectrophotomer. This apparatus had been previously validated by good agreement of experimental measurements with predictive mathematical models of oxygen transport for either erythrocyte suspensions or hemoglobin solutions. The experimental methodology was extended to measurement of oxygen transport in erythrocyte/hemoglobin solutions. The hemoglobin solutions consisted of either purified or gluteraldehyde polymerized bovine hemoglobin. Dose-response plots were generated by varying the extracellular to intracellular hemoglobin ratio while holding the overall hemoglobin concentration constant. Measurements were also made on unmixed erythrocyte suspensions and hemoglobin solutions to generate limiting cases for comparison. Direct comparison of experimental results showed that both types of hemoglobin solutions were substantially more efficient than erythrocyte suspension in uptake and release of oxygen. Increased extracellular hemoglobin concentration increased oxygen transport efficiency for both uptake and release, even when total hemoglobin concentration was held constant, When only 10% of the total hemoglobin was extracellular, approximately half of the increased efficiency of pure hemoglobin solutions was reached. When 50% of the total hemoglobin was extracellular, the increased efficiency was virtually equal to that of pure hemoglobin solutions. (C) 1998 Academic Press.