Nano-imaging of the lymph network structure with quantum dots
Nano-imaging of the lymph network structure with quantum dots
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DOI：
10.1088/0957-4484/21/18/185103
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发表时间：
2010-05-07
期刊：
影响因子：
3.5
通讯作者：
Ohuchi, Noriaki
中科院分区：
文献类型：
--
作者：
Hikage, Makoto;Gonda, Kohsuke;Ohuchi, Noriaki
前哨淋巴结诊断有助于癌症外科手术策略的制定。在淋巴结转移过程中，癌细胞首先通过淋巴流到达前哨淋巴结(SLN)。为了有效地进行SLN活检，在与肿瘤部位相连的SLN中以高灵敏度检测癌细胞是很重要的。在这里，我们提出了一种方法，以可视化的高危区域在SLN的淋巴结转移，并具有很高的准确性。将明亮的荧光纳米粒子量子点(Qds)注射到猪的胃肠壁，用腹腔镜设备在SLN中特异性地检测到它们的信号。共聚焦显微镜下的单粒子成像显示，量子点在SLN内呈不均匀分布，它们的分布标志着传入淋巴管的流入轨迹，是淋巴结转移的起点。此外，我们开发了一种使用细胞标记共轭量子点的方法来显示SLN中的特定细胞，这表明该方法可以应用于使用肿瘤特异性分子共轭量子点来检测前哨淋巴结中的癌细胞。这些结果表明，我们的方法可能会显着提高SLN中肿瘤转移的检测率。
Sentinel lymph node diagnosis contributes to operative strategy in cancer surgery. During lymph node metastasis, cancer cells first reach the sentinel lymph node (SLN) via lymph flow. To perform SLN biopsy effectively, it is important that cancer cells are detected with high sensitivity in SLN connected to the tumor site. Here we present a method to visualize a high-risk area in the SLN for lymph node metastasis with a high degree of accuracy. Quantum dots (QDs), bright fluorescent nanoparticles, were endoscopically injected into the gastrointestinal wall of pigs, and their signal was specifically detected in the SLN with a laparoscopic device. Single-particle imaging under a confocal microscope showed that the QDs were distributed heterogeneously in the SLN and that their distribution marked the inflow locus of afferent lymphatic vessels where lymph node metastasis begins. Moreover, we developed a method using cellular marker conjugated QDs that visualizes specific cells in SLNs, suggesting that this method can be applied for the detection of cancer cells in sentinel lymph nodes using tumor-specific-molecular conjugated QDs. These results show that our method might significantly increase the detection rate of cancer metastasis in SLNs.