Effects of dose, pH and osmolarity on intranasal absorption of recombinant human erythropoietin in rats.
Effects of dose, pH and osmolarity on intranasal absorption of recombinant human erythropoietin in rats.
复制标题
剂量、pH 和渗透压对重组人促红细胞生成素大鼠鼻内吸收的影响。
DOI：
10.1248/bpb.18.734
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发表时间：
1995
影响因子：
2
通讯作者：
T. Nagai
中科院分区：
文献类型：
--
作者：
N. Shimoda;Y. Maitani;Y. Machida;T. Nagai
用雄性Wistar大鼠研究了剂量、pH和渗透压对重组人促红细胞生成素(REPO)溶液鼻腔吸收的影响。鼻腔给药通过测量染色血涂片上红细胞的循环网织红细胞百分比(涂片法)和使用微细胞计数器测量剩余的循环网织红细胞计数(Sysmex法)来评价。这两个结果都表明，REPO溶液在一次鼻腔给药后，在没有促进剂的大鼠的鼻黏膜中被吸收。用涂片法和Sysmex法估算，鼻腔给药与静脉给药的药理利用度分别为7%和4%。在低pH、低渗甘露醇溶液中药理活性增强。
The effects of dose, pH and osmolarity on the intranasal absorption of a recombinant human erythropoietin (rEPO) solution were studied in male Wistar rats. The intranasal administration of rEPO was evaluated by measuring percentage circulating reticulocytes of red blood cells on a stained blood smear (smear method), and also by measuring residual circulating reticulocyte counts using a microcell counter (sysmex method). Both results suggest that rEPO solution was absorbed through the nasal mucosa of rats without enhancers after a single intranasal administration. The pharmacological availabilities of rEPO after intranasal administration compared with intravenous administration were about 7% and 4%, when estimated by smear method and sysmex method, respectively. The pharmacological activity was enhanced in low pH and hypotonic mannitol solution.