Bone regeneration by grafting of cultured human bone.
Bone regeneration by grafting of cultured human bone.
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DOI：
10.1089/1076327041348482
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发表时间：
2004-08
影响因子：
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通讯作者：
T. Yoshikawa;H. Ohgushi;K. Ichijima;Y. Takakura
中科院分区：
文献类型：
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作者：
T. Yoshikawa;H. Ohgushi;K. Ichijima;Y. Takakura
从27名骨科患者（8名男性和19名女性，平均年龄为56.1岁[范围，17 - 76岁]）的髂骨采集3 mL骨髓样本，然后在标准培养基（含15%胎牛血清的最低必需培养基）中培养。从这27例患者中随机选择的所有7例患者中，通过扫描电镜和生化分析证明骨髓间充质细胞具有显著的体外成骨能力。在所有27例病例中，为了研究这种人培养骨的体内成骨潜力，将多孔陶瓷用骨髓细胞浸渍并在成骨培养基（补充有β-甘油磷酸钠、维生素C磷酸盐和地塞米松的标准培养基）中传代培养。传代培养3周后，将培养骨/多孔陶瓷的培养人工骨移植到裸鼠腹腔内。组织学和生物化学（碱性磷酸酶活性和人骨钙素）检测表明，培养的人工骨具有明显的骨再生能力。这一结果表明，人骨髓细胞的骨再生能力可能不依赖于年龄，如果能够成功制备适当的培养骨髓细胞，培养人工骨可能用于骨再生治疗。
A 3-mL sample of bone marrow was collected from the iliac bones of 27 orthopedic patients (8 men and 19 women with a mean age of 56.1 years [range, 17 to 76 years]), followed by culture in standard culture medium (minimal essential medium containing 15% fetal bovine serum). In all 7 patients randomly selected from these 27 patients, significant in vitro osteogenic ability of marrow mesenchymal cells was demonstrated by scanning electron microscopy and biochemical analyses. In all 27 cases, to investigate the in vivo osteogenic potential of this human cultured bone, porous ceramics were impregnated with marrow cells and subcultured in osteogenic culture medium (standard medium supplemented with sodium beta-glycerophosphate, vitamin C phosphate, and dexamethasone). After 3 weeks of subculture, the cultured artificial bones of the cultured bone/porous ceramics were grafted into the abdominal cavity of nude mice. Histological and biochemical (alkaline phosphatase activity and human osteocalcin) examinations indicated that the cultured artificial bone possessed significant ability to regenerate bone. This result suggests that the bone-regenerating ability of human marrow cells may not depend on age, and that cultured artificial bone may be useful for bone regeneration treatment if appropriate cultured marrow cells can be successfully prepared.