Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.
Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.
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DOI：
10.1007/s13205-010-0001-5
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发表时间：
2011-07-01
期刊：
影响因子：
2.8
通讯作者：
Venkatasubramanian, Padma
中科院分区：
文献类型：
--
作者：
Balasubramani, Subramani Paranthaman;Goraya, Gurinder Singh;Venkatasubramanian, Padma
小檗的茎。（小檗科）在南亚传统医学中被用作眼部护理、皮肤病、黄疸、风湿病和糖尿病配方的关键成分。枸杞子和枸杞子。都可以作为马蹄草的等价物进行交易。传统的宏观形态学和显微镜检查不能帮助严格区分这三个物种。利用通用引物扩增基因组DNA内完整转录间隔区（ITS1、5.8S rRNA和ITS2）并测序，形成DNA标记。所建立的标记可有效、可靠地鉴别枸杞子、亚洲枸杞子和枸杞子。这些方法可作为原料药质量控制的分子生药学工具。
The stems of Berberis aristata DC. (Berberidaceae) are used in the South Asian traditional medicine as a key ingredient in formulations for eye care, skin diseases, jaundice, rheumatism and also in diabetes. B. lycium Royle and B. asiatica Roxb. are traded as equivalents of B. aristata. Conventional macro-morphology and microscopic examination does not aid in critically distinguishing the three species. DNA markers were developed by amplifying and sequencing the complete internal transcribed spacer region (ITS1, 5.8S rRNA and ITS2) from the genomic DNA, using universal primers. The markers developed are efficient and reliable in authenticating B. aristata, B. asiatica and B. lycium. These are useful as molecular pharmacognostic tool in quality control of raw drugs.