Sites of renal pain processing in the rat spinal cord.: A c-fos study using a percutaneous method to perform ureteral obstruction
Sites of renal pain processing in the rat spinal cord.: A c-fos study using a percutaneous method to perform ureteral obstruction
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DOI：
10.1016/s0165-1838(97)00105-7
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发表时间：
1997-12-03
期刊：
影响因子：
--
通讯作者：
Coimbra, A
中科院分区：
文献类型：
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作者：
Avelino, A;Cruz, F;Coimbra, A
通过绘制由于梗阻引起的急性输尿管扩张后表达 c-Sos 的脊髓神经元，研究了大鼠脊髓中肾痛处理的部位。提出了一种新的实验模型。将尼龙结松散地放置在输尿管周围，并将线的末端穿过腹膜后壁伸出。 8天后，当由于腹部伤口的伤害性输入和肠道操作而导致的所有c-fos表达消失时，拉动尼龙末端以产生输尿管闭塞，C-fos激活发生在脊髓节段T10-L4，在L1-L2处达到峰值。激活的神经元集中在 I 层、外侧 IV-V、内侧 VIT 和 X 中。而在 I 层中，几乎所有 Fos 免疫反应性细胞都位于同侧，而在更深的层中，60% 的细胞位于扩张输尿管的同侧，40% 位于扩张的输尿管的对侧。有人提出，引起有意识疼痛感知的肾脏伤害性输入是通过同侧 I 层传递的，而触发自主神经反射的输入可能主要在深层处理（同侧和对侧）。 (C) 1997 Elsevier Science B.V.
The sites of renal pain processing in the rat spinal cord were studied by mapping the spinal cord neurons expressing c-Sos after acute ureteral distension due to obstruction. A new experimental model is presented. A nylon knot was loosely placed around the ureter and the ends of the thread exteriorized through the retroperitoneal wall. Eight days later, when all c-fos expression due to nociceptive input from the abdominal wound and the manipulation of the intestines had disappeared, the nylon ends were pulled to produce ureteral occlusion, C-fos activation occurred at spinal segments T10-L4 with a peak at L1-L2. The activated neurons were concentrated in laminae I, lateral IV-V, medial VIT and X. While in lamina I nearly all Fos-immunoreactive cells were ipsilateral, in the deeper laminae taken together 60% cells were ipsilateral and 40% contralateral to the distended ureter. It is suggested that renal nociceptive input giving rise to conscious pain perception is transmitted through ipsilateral lamina I, whereas input triggering autonomic reflexes may be mainly processed, ipsi-and contralaterally, in the deep laminae. (C) 1997 Elsevier Science B.V.