A glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode

A glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode
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DOI:
10.1016/j.bios.2004.08.005
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发表时间:
2005-05-15
影响因子:
12.6
通讯作者:
KuaYou, J
KuaYou, J
中科院分区:
工程技术1区
文献类型:
--
作者:
Lim, SH;Wei, J;KuaYou, J

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采用电沉积法将葡萄糖氧化酶(GOx)和钯纳米颗粒共沉积到Nation增溶的碳纳米管(CNT)膜上。共沉积的Pd-GOx-Nafion CNT生物电极保留其生物催化活性,并提供酶促释放的H2 O2的有效氧化和还原,从而实现快速和灵敏的葡萄糖定量。Pd-GOx电沉积与Nafion增溶的CNT的组合提高了传感器的存储时间和性能。一个额外的Nafion涂层被用来消除常见的干扰物,如尿酸和抗坏血酸。所制备的Pd-GOx-Nafion CNT葡萄糖生物传感器表现出线性响应高达12 mM的葡萄糖和0.15 mM的检测限(S/N=3)。(c)2004 Elsevier B. V.保留所有权利。
Electrodeposition was used for the co-deposition of glucose oxidase (GOx) enzymes and palladium nanoparticles onto a Nation-solubilized carbon nanotube (CNT) film. The co-deposited Pd-GOx-Nafion CNT bioelectrode retains its biocatalytic activity and offers an efficient oxidation and reduction of the enzymatically liberated H2O2, allowing for fast and sensitive glucose quantification. The combination of Pd-GOx electrodeposition with Nafion-solubilized CNTs enhances the storage time and performance of the sensor. An extra Nafion coating was used to eliminate common interferents such as uric and ascorbic acids. The fabricated Pd-GOx-Nafion CNT glucose biosensor exhibits a linear response up to 12 mM glucose and a detection limit of 0.15 mM (S/N=3). (c) 2004 Elsevier B.V. All rights reserved.