Transcriptome Analysis of a Wheat Near-Isogenic Line Pair Carrying Fusarium Head Blight-Resistant and -Susceptible Alleles
Transcriptome Analysis of a Wheat Near-Isogenic Line Pair Carrying Fusarium Head Blight-Resistant and -Susceptible Alleles
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DOI：
10.1094/mpmi-22-11-1366
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发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Muehlbauer, Gary J.
中科院分区：
文献类型：
--
作者：
Jia, Haiyan;Cho, Seungho;Muehlbauer, Gary J.
小麦赤霉病主要由禾谷镰刀菌引起，严重影响小麦和大麦的产量和品质。对携带3BS抗赤霉病数量性状基因座(Fhb1)抗性或感病等位基因的小麦近等基因系进行了病情严重程度、脱氧雪腐镰刀菌烯醇(DON)、真菌生物量和转录本积累的检测。Fhb1限制了疾病症状的传播，但不能抵抗最初的感染或最初的DON积累。小麦在禾谷镰刀菌侵染过程中表现出对大量基因转录本的诱导和抑制。此外，在Fhb1基因座上携带抗性和感病等位基因的植株中，转录积累的一般时间存在差异，并检测到14个小麦基因转录本，它们在抗感等位基因之间存在积累差异。这些结果表明，这些可能是区分抗性和感病互作的寄主反应。小麦-F。Graminearum和大麦F。禾本科之间的相互作用揭示了一大套保守的转录积累模式。然而，我们也检测到在小麦中被抑制而在大麦中不被抑制的基因转录本。根据病害症状、转录积累数据以及大麦和小麦寄主对禾谷镰刀菌侵染反应的比较分析，我们建立了小麦和大麦与禾谷镰刀菌相互作用的综合模型。
Fusarium head blight (FHB), caused primarily by Fusarium graminearum, decreases grain yield and quality in wheat and barley. Disease severity, deoxynivalenol (DON), fungal biomass, and transcript accumulation were examined in a wheat near-isogenic line pair carrying either the resistant or susceptible allele for the chromosome 3BS FHB-resistance quantitative trait locus (Fhb1). Fhb1 restricts spread of disease symptoms but does not provide resistance to initial infection or initial DON accumulation. Wheat exhibits both induction and repression of large sets of gene transcripts during F. graminearum infection. In addition, a difference in the general timing of transcript accumulation in plants carrying either the resistant or susceptible allele at the Fhb1 locus was detected, and 14 wheat gene transcripts were detected that exhibited accumulation differences between the resistant and susceptible alleles. These results indicate that these may be host responses that differentiate the resistant from the susceptible interaction. Comparative analysis of the wheat-F. graminearum and the barley-F. graminearum interactions revealed a large set of conserved transcript accumulation patterns. However, we also detected gene transcripts that were repressed in wheat but not in barley. Based on the disease symptoms, transcript accumulation data, and comparative analysis of the barley and wheat host response to F. graminearum infection, we developed an integrated model for the interactions of wheat and barley with F. graminearum.