A KINETIC-ANALYSIS OF DROSOPHILA-MELANOGASTER DOPA CARBOXYLASE
A KINETIC-ANALYSIS OF DROSOPHILA-MELANOGASTER DOPA CARBOXYLASE
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DOI：
10.1016/0167-4838(86)90005-1
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发表时间：
1986-03-07
期刊：
影响因子：
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通讯作者：
SMARRELLI, J
中科院分区：
文献类型：
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作者：
BLACK, BC;SMARRELLI, J
研究了黑腹果蝇（Drosophila melanogaster）多巴脱羧酶（3,4-二羟基-l -苯丙氨酸羧化酶，EC 4.4.1.28）的动力学机制。基于初速度和产物抑制的研究，提出了多巴脱羧酶的有序反应。这一动力学机制是在测量酶活性和儿茶酚胺池在果蝇的背景下解释的。1(2)amd基因紧邻编码多巴脱羧酶（Ddc）的基因，决定了对。α的超敏性。果蝇中的甲基多巴。多巴脱羧酶不能使α脱羧。-甲基多巴，因此不会产生能够抑制1(2)amd基因功能的有毒产物。我们提出1(2)amd基因参与了一个未知的儿茶酚胺通路，涉及多巴，但不涉及多巴胺。
The kinetic mechanism of dopa decarboxylase (3,4-dihydroxy-L-phenylalanine carboxy-lyase, EC 4.4.1.28) was investigated in Drosophila melanogaster. Based on initial velocity and product inhibition studies, an ordered reaction is proposed for dopa decarboxylase. This kinetic mechanism is interpreted in the context of measured enzyme activities and the catecholamine pools in Drosophila. The 1(2)amd gene is immediately adjacent to the gene coding for dopa decarboxylase (Ddc) and determines hypersensitivity to .alpha.-methyldopa in Drosophila. Dopa decarboxylase does not decarboxylate .alpha.-methyldopa and hence does not generate a toxic product capable of inhibiting 1(2)amd gene function. We propose that the 1(2)amd gene is involved with an unknown catecholamine pathway involving dopa but not dopamine.