Neural remodeling following experimental surgery of the rabbit cornea.
Neural remodeling following experimental surgery of the rabbit cornea.
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DOI：
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发表时间：
1983-08
影响因子：
4.4
通讯作者：
A. J. Rózsa;R. B. Guss;R. Beuerman
中科院分区：
文献类型：
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作者：
A. J. Rózsa;R. B. Guss;R. Beuerman
角膜的损伤导致神经支配的广泛损伤，必须修复以恢复其正常的结构和功能。在幼年白化兔角膜上制造了四种实验性创面：180度穿透性包膜周围切口、4 mm中央圆形角膜切除术和角膜切开术、放射状角膜切开术。在缘缘切口后，失神经的一半角膜主要通过穿透缘缘瘢痕组织的再生神经进行再神经支配。来自角膜受神经支配部分的新神经生长对无神经支配区域的贡献较小。发现非穿透性手术后神经的再生反应是一个双相过程。在第一阶段，所有被伤口边界包围区域内的神经在短时间内退行性变，出现垂直于伤口边缘的长、大口径、密集的神经突。神经突起源于离伤口边缘一定距离的完整的上皮下神经丛。第二阶段是由伤口导向神经突的退化和伴随的第二代神经突的出现而开始的。这些新的神经突起源于伤口边缘或附近再生的上皮下轴突的横切残端。第二波神经突的斜向分布与正常角膜的基底带相似。非穿透性损伤过程表现出类似的神经重塑原理。在两种类型的角膜切除术中，神经末梢在伤口内以持久和密集的神经瘤样排列终止，而在角膜切除术中，神经末梢继续向角膜中心生长。
Wounding of the cornea results in extensive damage to the innervation that must be repaired to restore its normal structure and function. Four types of experimental wounds were produced in the corneas of young albino rabbits: 180 degrees penetrating perilimbal incisions, 4-mm central circular keratectomies and keratotomies, and radial keratotomies. Following perilimbal incisions, the denervated half of the cornea was reinnervated primarily by regenerating nerves that penetrated the limbal scar tissue. New neural growth from the innervated portion of the cornea provided a minor contribution to the denervated area. The regenerative response of the nerves following nonpenetrating procedures was found to be a biphasic process. In the first phase, a short period of degeneration of all nerves within the area enclosed by the wound boundary overlapped in time with the appearance of long, large caliber, dense neurites that coursed perpendicularly to the wound margins. The neurites originated from the intact subepithelial plexus at some distance from the wound margins. The second phase was initiated by the degeneration of the wound-oriented neurites and the concomitant appearance of a second generation of neurites. These new neurites originated from the transected stumps of the regenerating subepithelial axons at or near the wound margins. The oblique disposition of the second wave of neurites was similar to that of basal leashes in normal corneas. Nonpenetrating wounding procedures exhibited similar neural remodeling principles. In both types of keratotomies, nerve endings terminated within the wound in enduring and densely packed neuroma-like arrangements, while in keratectomies, nerve endings continued to grow toward the center of the cornea.