Phosphorylation at Ser65 modulates ubiquitin conformational dynamics.
Phosphorylation at Ser65 modulates ubiquitin conformational dynamics.
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Ser65 的磷酸化可调节泛素构象动力学。
DOI：
10.1016/j.str.2023.05.006
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Lau,AlbertY
中科院分区：
文献类型：
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作者：
Yovanno,RemyA;Yu,Alvin;Wied,TylerJ;Lau,AlbertY
Ser65 处的泛素磷酸化会增加罕见的 C 末端回缩 (CR) 构象的数量。主要泛素和 CR 泛素构象之间的转变对于促进线粒体降解至关重要。然而，Ser65 磷酸化 (pSer65) 泛素的主要构象和 CR 构象相互转换的机制仍未解决。在这里，我们使用弦法和轨迹群进行全原子分子动力学模拟，以计算这两个构象异构体之间的最低自由能路径。我们的分析揭示了 Bent 中间体的存在，其中 β5 链的 C 端残基转变为类似于 CR 构象，而 pSer65 保留了类似于 Major 构象的接触。这种稳定的中间体在经过调节的元动力学计算中得到了重现，但对于破坏与 pSer65 接触的 Gln2Ala 突变体来说不太稳定。最后，动态网络模型表明，从主要构象到 CR 构象的转变涉及 pSer65 附近的残基与相邻 β1 链的解偶联。
Ubiquitin phosphorylation at Ser65 increases the population of a rare C-terminally retracted (CR) conformation. Transition between the Major and CR ubiquitin conformations is critical for promoting mitochondrial degradation. The mechanisms by which the Major and CR conformations of Ser65-phosphorylated (pSer65) ubiquitin interconvert, however, remain unresolved. Here, we perform all-atom molecular dynamics simulations using the string method with swarms of trajectories to calculate the lowest free-energy path between these two conformers. Our analysis reveals the existence of a Bent intermediate in which the C-terminal residues of the β5 strand shift to resemble the CR conformation, while pSer65 retains contacts resembling the Major conformation. This stable intermediate was reproduced in well-tempered metadynamics calculations but was less stable for a Gln2Ala mutant that disrupts contacts with pSer65. Lastly, dynamical network modeling reveals that the transition from the Major to CR conformations involves a decoupling of residues near pSer65 from the adjacent β1 strand.