The MADS transcription factor CmANR1 positively modulates root system development by directly regulating CmPIN2 in chrysanthemum.
The MADS transcription factor CmANR1 positively modulates root system development by directly regulating CmPIN2 in chrysanthemum.
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MADS转录因子CmANR1通过直接调节菊花中的CmPIN2正向调节根系发育
DOI：
10.1038/s41438-018-0061-y
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发表时间：
2018
影响因子：
8.7
通讯作者：
Zheng CS
中科院分区：
文献类型：
--
作者：
Sun CH;Yu JQ;Duan X;Wang JH;Zhang QY;Gu KD;Hu DG;Zheng CS
植物根系对许多生理过程至关重要，包括水分和养分的吸收。MADS-box转录因子（MADS-box transcription factor， TF）是植物根系发育的重要调控因子；然而，潜在的机制在很大程度上是未知的，包括菊花。本研究发现，菊花MADS-box TF基因CmANR1的过表达促进了菊花不定根（AR）和侧根（LR）的发育。全转录组测序分析显示，与野生型植物相比，cmanr1转基因菊花植物的根中存在一系列差异表达的单基因（DEGs）。通过基因本体（GO）术语和生化途径京都基因与基因组百科全书（KEGG）富集分析对这些DEGs进行功能注释，表明CmANR1 TF具有“DNA结合”和“催化”活性，并参与“植物激素信号转导”。染色质免疫沉淀-聚合酶链反应（ChIP-PCR）和凝胶电泳迁移转移试验（EMSA）均表明CmPIN2通过CmANR1直接结合到识别位点CArG-box基序上。最后，萤火虫荧光素酶成像实验证明了CmANR1在体内对CmPIN2的转录激活。总的来说，我们的研究结果为MADS-box TF CmANR1通过直接调节菊花生长素转运基因CmPIN2调控AR和LR发育的机制提供了新的见解。
Plant root systems are essential for many physiological processes, including water and nutrient absorption. MADS-box transcription factor (TF) genes have been characterized as the important regulators of root development in plants; however, the underlying mechanism is largely unknown, including chrysanthemum. Here, it was found that the overexpression of CmANR1, a chrysanthemum MADS-box TF gene, promoted both adventitious root (AR) and lateral root (LR) development in chrysanthemum. Whole transcriptome sequencing analysis revealed a series of differentially expressed unigenes (DEGs) in the roots of CmANR1-transgenic chrysanthemum plants compared to wild-type plants. Functional annotation of these DEGs by alignment with Gene Ontology (GO) terms and biochemical pathway Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that CmANR1 TF exhibited “DNA binding” and “catalytic” activity, as well as participated in “phytohormone signal transduction”. Both chromatin immunoprecipitation–polymerase chain reaction (ChIP-PCR) and gel electrophoresis mobility shift assays (EMSA) indicated the direct binding of CmPIN2 to the recognition site CArG-box motif by CmANR1. Finally, a firefly luciferase imaging assay demonstrated the transcriptional activation of CmPIN2 by CmANR1 in vivo. Overall, our results provide novel insights into the mechanisms of MADS-box TF CmANR1 modulation of both AR and LR development, which occurs by directly regulating auxin transport gene CmPIN2 in chrysanthemum.