CELL-SURFACE ANTIGENS OF HUMAN BLADDER-CANCER DEFINED BY MOUSE MONOCLONAL-ANTIBODIES
CELL-SURFACE ANTIGENS OF HUMAN BLADDER-CANCER DEFINED BY MOUSE MONOCLONAL-ANTIBODIES
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DOI：
10.1073/pnas.81.1.224
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
OLD, LJ
中科院分区：
文献类型：
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作者：
FRADET, Y;CORDONCARDO, C;OLD, LJ
用培养的人膀胱癌细胞或膀胱乳头状瘤细胞裂解液免疫小鼠获得单克隆抗体。他们通过对培养细胞的血清学分析和对正常和肿瘤组织中抗原分布的研究确定了11种不同的抗原系统。这些抗原中最受限制的Om5定义了膀胱肿瘤的一个子集。Om5在正常膀胱尿路上皮或任何其他正常或恶性组织中未检测到。T101和JP165也是膀胱癌的亚群标志物，在正常组织中检测不到。T16、T43、T87和J143（在许多培养细胞上代表的抗原）存在于正常泌尿道的特定区域和其它正常和恶性细胞类型的独特范围中，T16在皮肤、外宫颈和食管复层上皮中的表达。T138抗原也是培养细胞系的共同特征，但其在正常组织切片中的表达仅限于内皮细胞。T110在培养的细胞上表现不佳，但可以在培养上清液中检测到。T110在正常组织中的定位表明它是细胞外基质的组分。该系列抗体检测到的所有决定簇均为热不稳定的，与A、B、H、I、刘易斯血型抗原无关。六种抗体免疫沉淀来自放射性标记的细胞裂解物的糖蛋白：AbT16（MW 48，000和42，000）、AbT87（MW 60，000）、AbT43（MW 85，000）、AbJ143（MW 140，000、120，000和30，000）和AbT110（MW 240，000）。
Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or lysates of bladder papilloma. They identify 11 distinct antigenic systems as defined by serological analysis of cultured cells and studies of antigen distribution in normal and neoplastic tissues. The most restricted of these antigens, Om5, defines a subset of bladder tumors. Om5 is not detected in normal bladder urothelium or in any other normal or malignant tissue. T101 and JP165 are also subset markers for bladder cancer that are not detected in normal tissues. T16, T43, T87 and J143 (antigens represented on many cultured cells) are found in specific areas of the normal urinary tract and in a distinctive range of other normal and malignant cell types, e.g., T16 expression in pluristratified epithelium of skin, exocervix, and esophagus. T138 antigen is also a common feature of cultured cell lines, but its expression in sections of normal tissues is restricted to endothelial cells. T110 is poorly represented on cultured cells but can be detected in culture supernatants. Localization of T110 in normal tissues showed that it is a component of the extracellular matrix. All determinants detected by this series of antibodies are heat labile and not related to A, B, H, I, Lewis blood group antigens. Six of the antibodies immunoprecipitated glycoproteins from radiolabeled cell lysates: AbT16 (MW 48,000 and 42,000), AbT87 (MW 60,000), AbT43 (MW 85,000), AbJ143 (MW 140,000, 120,000 and 30,000), and AbT110 (MW 240,000).