SEPARATION OF NUCLEIC-ACIDS BY CAPILLARY ELECTROPHORESIS IN CELLULOSE SOLUTIONS WITH MONO-INTERCALATING AND BIS-INTERCALATING DYES
SEPARATION OF NUCLEIC-ACIDS BY CAPILLARY ELECTROPHORESIS IN CELLULOSE SOLUTIONS WITH MONO-INTERCALATING AND BIS-INTERCALATING DYES
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DOI：
10.1021/ac00079a035
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发表时间：
1994-04-01
影响因子：
7.4
通讯作者：
MORRIS, MD
中科院分区：
文献类型：
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作者：
KIM, YS;MORRIS, MD
在含(羟丙甲基)纤维素的缓冲溶液中，研究了双单插层染料对毛细管电泳法分离双链DNA的影响。在较宽的染料和聚合物浓度范围内，双嵌入剂乙二胺高二聚体1(ethD-1)和乙二胺高二聚体2(ethD-2)对phiX 174 DNA HaeIII酶切获得了较宽的条带和不完全分离。上述结果归因于每个片段存在几个分子内染料/DNA络合物。对于ethD-2/DNA复合体，观察到了超出预期片段数目的额外条带，但对于ethD-1/DNA复合体则没有观察到。这些额外的条带归因于分子间二聚体的形成。使用单体插层染料乙锭和丙啶，可以完全分离phiX 174 DNA HaeIII酶的所有片段。用低功率绿色He-Ne激光器(543.6 nm)对上述染料进行激光诱导荧光检测是令人满意的。
The effects of bis- and mono-intercalating dyes on the capillary electrophoretic separation of double-stranded DNA have been investigated in buffers containing (hydroxypropylmethyl)-cellulose. Broad bands and incomplete separations of phiX 174 DNA HaeIII digest were obtained with the bis-intercalators ethidium homodimer 1 (EthD-1) and ethidium homodimer 2 (EthD-2), under a wide range of dye and polymer concentrations. The above results were attributed to the presence of several intramolecular dye/DNA complexes for each fragment. For EthD-2/DNA complexes, but not for EthD-1/DNA complexes, additional bands beyond the expected number of fragments were observed. The extra bands were attributed to formation of intermolecular dimers. Use of the monomeric intercalating dyes, ethidium and propidium, allowed complete separation of all fragments of phiX 174 DNA HaeIII digest. A low-power green He-Ne laser (543.6 nm) was shown to be satisfactory for laser-induced fluorescence detection with any of these dyes.