Microbiome Survey of the Inflamed and Noninflamed Gut at Different Compartments Within the Gastrointestinal Tract of Inflammatory Bowel Disease Patients
Microbiome Survey of the Inflamed and Noninflamed Gut at Different Compartments Within the Gastrointestinal Tract of Inflammatory Bowel Disease Patients
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DOI：
10.1097/mib.0000000000000684
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发表时间：
2016-04-01
影响因子：
4.9
通讯作者：
Bernstein, Charles N.
中科院分区：
文献类型：
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作者：
Forbes, Jessica D.;Van Domselaar, Gary;Bernstein, Charles N.
背景：我们旨在对比克罗恩病（CD）和溃疡性结肠炎（UC）的粘膜微生物群。研究方法：我们通过比较炎症性肠病（IBD）患者炎症和非炎症粘膜的细菌群落，并通过分析不同肠道隔室（回肠，盲肠，结肠中段和直肠）的微生物群组成，评估了局部生态失调的概念。我们进行了16S rDNA测序分析种群结构。使用mothur进行读段的质量控制和操作分类单元分类，其中在R软件包中执行统计分析，RISseq。结果如下：在CD（或UC）内炎症粘膜与非炎症粘膜的比较中，或在CD（或UC）内比较不同肠道隔室时，任何门或属均无变化。CD和UC之间的炎症和非炎症粘膜之间存在差异：在门水平上对炎症IBD肠道的分析表明，在炎症CD粘膜中比在炎症UC粘膜中更频繁地检测到拟杆菌（P = 0.002）和梭菌（P < 0.05）。相反，变形菌门和厚壁菌门（P < 0.05）更频繁地观察到在发炎的UC粘膜。在属的水平上，粪杆菌属（P
Background: We aimed to contrast the mucosal microbiota in Crohn's disease (CD) and ulcerative colitis (UC). Methods: We assessed the concept of localized dysbiosis by comparing the bacterial communities of inflamed and noninflamed mucosa of patients with inflammatory bowel disease (IBD) and by analysis of the microbiota composition at distinct gut compartments (ileum, cecum, mid-colon, and rectum). We performed 16S rDNA sequencing to analyze population structures. Quality control and operational taxonomic unit classification of reads were performed using mothur with statistical analyses executed in the R package, phyloseq. Results: There was no variation in any phyla or genera comparing inflamed to noninflamed mucosa within CD (or UC) or when comparing different gut compartments within CD (or UC). There were differences between the inflamed and noninflamed mucosa between CD and UC: analysis of the inflamed IBD gut at the phylum level indicated that Bacteroidetes (P = 0.002) and Fusobacteria (P < 0.05) were detected more frequently in inflamed CD mucosa than in inflamed UC mucosa. Conversely, Proteobacteria and Firmicutes (P < 0.05) were more frequently observed in the inflamed UC mucosa. At the genus level, the abundance of Faecalibacterium (P