Artemin has potent neurotrophic actions on injured C-fibres
Artemin has potent neurotrophic actions on injured C-fibres
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DOI：
10.1111/j.1529-8027.2006.00106.x
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发表时间：
2006-12-01
影响因子：
3.8
通讯作者：
McMahon, Stephen B.
中科院分区：
文献类型：
--
作者：
Bennett, David L. H.;Boucher, Timothy J.;McMahon, Stephen B.
在这项研究中，我们研究了artenin（ARTN），胶质细胞源性神经营养因子（GDNF）家族的神经营养因子之一，对C-纤维神经损伤后在成年大鼠的影响。GDNF家族受体α（GFR α）3是ARTN受体的配体结合结构域，在34%的背根神经节（DRG）细胞中表达，主要在C-纤维的肽能群体中表达，并在一定比例的同种凝集素B4（IB 4）结合群体中表达。有趣的是，只有30%的表达GFR α 3的DRG细胞共表达RET（信号转导结构域）。与以前的研究一致，用ARTN治疗防止了许多神经损伤诱导的小直径DRG细胞的肽能和IB 4结合群体的组织化学变化，但不是大直径DRG细胞。此外，ARTN治疗维持C-纤维传导速度，和C-纤维诱发的P物质释放后，神经损伤的背角。辣椒素治疗后，ARTN也有保护作用，辣椒素治疗会产生选择性C纤维损伤。鉴于ARTN对C纤维的有效神经营养作用，因此它可能为神经损伤的治疗提供潜力，特别是在维持小纤维功能方面。
In this study, we have investigated the effects of artemin (ARTN), one of the glial cell line-derived neurotrophic factor (GDNF) family of neurotrophic factors, on C-fibres following nerve injury in the adult rat. GDNF family receptor alpha (GFR alpha) 3, the ligand binding domain of the ARTN receptor, is expressed in 34% of dorsal root ganglion (DRG) cells, predominantly in the peptidergic population of C-fibres and in a proportion of the isolectin B4 (IB4)-binding population. Interestingly, only 30% of GFR alpha 3-expressing DRG cells co-expressed RET (the signal transducing domain). In agreement with previous studies, treatment with ARTN prevented many of the nerve injury-induced changes in the histochemistry of both the peptidergic and the IB4-binding populations of small, but not large, diameter DRG cells. In addition, ARTN treatment maintained C-fibre conduction velocity, and C-fibre evoked substance P release within the dorsal horn following nerve injury. ARTN was also protective following capsaicin treatment, which produces selective C-fibre injury. Given the potent neurotrophic actions of ARTN on C-fibres, it may therefore provide potential for the treatment of nerve injury, particularly in the maintenance of small fibre function.