Lipopolysaccharide injected to pregnant mice affects behavior of their offspring in adulthood
Lipopolysaccharide injected to pregnant mice affects behavior of their offspring in adulthood
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DOI：
10.55782/ane-2011-1868
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发表时间：
2011-01-01
影响因子：
1.4
通讯作者：
Djavadian, Rouzanna L.
中科院分区：
文献类型：
--
作者：
Chlodzinska, Natalia;Gajerska, Monika;Djavadian, Rouzanna L.
我们研究了母体免疫反应对妊娠过程和成年后代行为的影响。对妊娠晚期小鼠（clay 16-17）注射脂多糖（LPS）。用高剂量LPS治疗妊娠小鼠导致胎儿吸收或死产。低剂量（100或300 μ g/kg） LPS处理的怀孕小鼠产下正常数量的幼崽。然而，后代的行为却发生了改变。注射300 μ g/kg LPS的成年小鼠比对照组小鼠在开阔场地的距离更远，在场地中心的时间更长。与对照组的雌鼠相比，这组雌鼠在高架迷宫中张开双臂的时间更长。Morris水迷宫实验结果显示，注射lps的雄性后代空间学习记忆功能受损。此外，在筑巢测试中，LPS挑战怀孕的成年小鼠筑巢质量较差，这表明存在海马功能障碍。这些发现表明，母亲在怀孕期间的细菌感染可能会改变后代成年后的行为。
We studied consequences of maternal immune response on the course of pregnancy and the behavior of adult offspring. Mice in late gestation (clay 16-17) were injected with lipopolysaccharide (LPS). Treatment of pregnant mice with high doses of LPS resulted in fetal resorption or stillbirths. Pregnant mice treated with low doses (100 or 300 mu g/kg) of LPS gave birth to normal numbers of pups. However, behavior of the offspring was altered. Adult offspring of dams injected at a dose of 300 mu g/kg of LPS traveled longer distances in the open field and spent more time in the central part of the arena, than mice in the control group. Female mice of this group spent more time in open arms of the elevated plus maze, in comparison to female control mice. Results of the Morris water maze test showed impairment of spatial learning and memory in male offspring born to LPS-injected dams. Furthermore, in the nest building test adult mice born from LPS challenged pregnancies constructed worse quality nests, which points to the presence of hippocampal dysfunction. These findings indicate that maternal bacterial infections during pregnancy may alter offspring behavior in adult life.