Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow
Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow
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DOI：
10.1242/jcs.00369
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发表时间：
2003-05-01
影响因子：
4
通讯作者：
Simmons, PJ
中科院分区：
文献类型：
--
作者：
Gronthos, S;Zannettino, ACW;Simmons, PJ
先前的研究已为成人骨髓基质干细胞（BMSSCs）或间充质干细胞的存在提供了证据。通过综合运用细胞分离技术，我们从成人骨髓中分离出了几乎同质的BMSSCs群体。BMSSCs缺乏白细胞和成熟基质成分的表型特征，在体内处于非增殖状态且组成性表达端粒酶活性。这种间充质干细胞群体表现出广泛的增殖能力，并在体外保留分化为骨、软骨和脂肪组织的能力。此外，克隆分析表明，单个BMSSC集落表现出在体内形成新骨的不同能力。这些数据与除造血系统干细胞之外还存在第二种骨髓干细胞群体的观点相符。我们新的筛选方案为产生纯化的BMSSCs群体提供了一种方法，可用于一系列不同的组织工程和基因治疗策略。
Previous studies have provided evidence for the existence of adult human bone marrow stromal stem cells (BMSSCs) or mesenchymal stem cells. Using a combination of cell separation techniques, we have isolated an almost homogeneous population of BMSSCs from adult human bone marrow. Lacking phenotypic characteristics of leukocytes and mature stromal elements, BMSSCs are noncycling and constitutively express telomerase activity in vivo. This mesenchymal stem cell population demonstrates extensive proliferation and retains the capacity for differentiation into bone, cartilage and adipose tissue in vitro. In addition, clonal analysis demonstrated that individual BMSSC colonies exhibit a differential capacity to form new bone in vivo. These data are consistent with the existence of a second population of bone marrow stem cells in addition to those for the hematopoietic system. Our novel selection protocol provides a means to generate purified populations of BMSSCs for use in a range of different tissue engineering and gene therapy strategies.