Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA
Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA
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DOI：
10.1159/000097427
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Macha, J.
中科院分区：
文献类型：
--
作者：
Krylov, V.;Tlapakova, T.;Macha, J.
利用荧光原位杂交结合酪胺信号放大(FISH-TSA)技术，将线粒体苹果酸脱氢酶2(Mdh2)基因定位于热带非洲爪哇染色体上。克隆并测序得到相应的基因片段。标记的探针与同源3号染色体长臂的亚着丝粒区域杂交。基因定位结果与先前定位的莱氏X.laevis Paralog的比较结果有力地表明，X.laevis的3号和8号染色体和热带X.laevis的3号染色体具有共同的进化起源。这是首次在热带管圆线虫染色体上发现单拷贝基因。FISHTSA方法用1kb标记的探针给出强烈的信号。版权所有(C)2007 S.Karger AG，巴塞尔
A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISHTSA method gives a strong signal with a 1-kb labeled probe. Copyright (c) 2007 S. Karger AG, Basel