Polydopamine and collagen coated micro-grated polydimethylsiloxane for human mesenchymal stem cell culture
Polydopamine and collagen coated micro-grated polydimethylsiloxane for human mesenchymal stem cell culture
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DOI：
10.1016/j.bioactmat.2019.02.002
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发表时间：
2019-12-01
影响因子：
18.9
通讯作者：
Zhao, Feng
中科院分区：
文献类型：
--
作者：
Sharma, Dhavan;Jia, Wenkai;Zhao, Feng
自然组织包含高度组织化的细胞结构。组织工程学的主要挑战之一是开发能够促进细胞在生理方向上生长的工程化组织结构。为了解决这一问题，微图案化聚二甲基硅氧烷(PDMS)衬底因其低微制造成本、高稳定性、良好的生物兼容性以及最重要的是引导细胞生长和图案化的能力而被广泛应用于细胞片工程中。然而，目前的PDMS表面改性方法要么需要复杂的过程，要么产生不均匀的表面涂层，导致产生质量较差的电池层。迫切需要一种简单有效的表面涂覆方法来提高所生成的电池层的均匀性和质量。因此，分析了一种快速、简单、廉价的表面包覆方法，该方法能够在不改变基本表面形貌的情况下，将含有或不含有胶原的聚多巴胺均匀地包覆在微细颗粒聚多巴胺(PDMS)上。进一步分析了Pd和/或胶原基表面涂层的形貌特征、硬度和细胞毒性。结果表明，基于Pd的包覆方法促进了细胞的定向和均匀生长，因此在细胞片工程以及完全生物组织的生物制造中具有广阔的应用前景。
Natural tissues contain highly organized cellular architecture. One of the major challenges in tissue engineering is to develop engineered tissue constructs that promote cellular growth in physiological directionality. To address this issue, micro-patterned polydimethylsiloxane (PDMS) substrates have been widely used in cell sheet engineering due to their low microfabrication cost, higher stability, excellent biocompatibility, and most importantly, ability to guide cellular growth and patterning. However, the current methods for PDMS surface modification either require a complicated procedure or generate a non-uniform surface coating, leading to the production of poor-quality cell layers. A simple and efficient surface coating method is critically needed to improve the uniformity and quality of the generated cell layers. Herein, a fast, simple and inexpensive surface coating method was analyzed for its ability to uniformly coat polydopamine (PD) with or without collagen on micro-grated PDMS substrates without altering essential surface topographical features. Topographical feature, stiffness and cytotoxicity of these PD and/or collagen based surface coatings were further analyzed. Results showed that the PD-based coating method facilitated aligned and uniform cell growth, therefore holds great promise for cell sheet engineering as well as completely biological tissue biomanufacturing.