Arsenic triggers the nitric oxide (NO) and S-nitrosoglutathione (GSNO) metabolism in Arabidopsis
Arsenic triggers the nitric oxide (NO) and S-nitrosoglutathione (GSNO) metabolism in Arabidopsis
复制标题
DOI：
10.1016/j.envpol.2012.03.012
复制
发表时间：
2012-07-01
影响因子：
8.9
通讯作者：
Corpas, Francisco J.
中科院分区：
文献类型：
--
作者：
Leterrier, Marina;Airaki, Morad;Corpas, Francisco J.
砷对环境的污染在许多国家构成了一个问题，它在粮食作物中的积累可能会给人类的健康带来并发症。活性氧(ROS)和活性氮(RNS)在不同程度上参与了高等植物对环境胁迫的响应机制。利用不同浓度的砷盐处理拟南芥幼苗，分析其生理生化指标，以确定ROS和RNS的代谢状况。砷引起生长参数的显著降低和脂质氧化的增加。伴随这些变化的是抗氧化酶和一氧化氮代谢的改变，其中NO含量、S-亚硝基谷胱甘肽还原酶活性和蛋白酪氨酸硝化显著升高，谷胱甘肽和S-亚硝基谷胱甘肽含量显著降低。我们的结果表明，500亩砷酸钼(ASV)引起拟南芥的亚硝基氧化胁迫，其中谷胱甘肽还原酶和GSNOR活性受到明显影响。(C)2012爱思唯尔有限公司。保留所有权利。
Environmental contamination by arsenic constitutes a problem in many countries, and its accumulation in food crops may pose health complications for humans. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved at various levels in the mechanism of responding to environmental stress in higher plants. Using Arabidopsis seedlings exposed to different arsenate concentrations, physiological and biochemical parameters were analyzed to determine the status of ROS and RNS metabolisms. Arsenate provoked a significant reduction in growth parameters and an increase in lipid oxidation. These changes were accompanied by an alteration in antioxidative enzymes and the nitric oxide (NO) metabolism, with a significant increase in NO content, S-nitrosoglutathione reductase (GSNOR) activity and protein tyrosine nitration as well as a concomitant reduction in glutathione and S-nitrosoglutathione (GSNO) content. Our results indicate that 500 mu M arsenate (AsV) causes nitro-oxidative stress in Arabidopsis, being the glutathione reductase and the GSNOR activities clearly affected. (C) 2012 Elsevier Ltd. All rights reserved.