GENOME AMPLIFICATION OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 IN CERVICAL CARCINOMAS IS RELATED TO THE RETENTION OF E1/E2 GENES
GENOME AMPLIFICATION OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 IN CERVICAL CARCINOMAS IS RELATED TO THE RETENTION OF E1/E2 GENES
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DOI：
10.1002/ijc.2910560506
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发表时间：
1994-03-01
影响因子：
6.4
通讯作者：
GARCIACARRANCA, A
中科院分区：
文献类型：
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作者：
BERUMEN, J;CASAS, L;GARCIACARRANCA, A
在不同临床阶段和组织学类型的 42 例 HPV16 和 21 例 HPV18 阳性宫颈癌样本中，分析了 HPV16 和 HPV18 病毒基因组的扩增水平（拷贝数/细胞）及其与 E1/E2 基因存在的相关性。通过斑点印迹杂交评估病毒拷贝数/细胞，并通过PCR和Southern印迹评估E1/E2基因的存在。 HPV18 阳性肿瘤中的拷贝数/细胞显着低于 HPV16 阳性肿瘤（分别为 23 +/- 8 和 457 +/- 191）。近一半的 HPV16 (43%) 与 HPV18 类似，分布在 50 个或更少拷贝的范围内，在 1 至 10 个拷贝的组中达到峰值，而其余 HPV16 (57%) 分布在 51 个或更多拷贝的组中，另一个峰值在 101 至 500 个拷贝的组中。E1/E2 区域在所有 HPV18 呈阳性的肿瘤中不存在，并且存在于 HPV18 阳性的肿瘤中。其中 64% HPV16 呈阳性。 E1/E2 阴性的 HPV16 肿瘤的病毒拷贝数 (17 +/- 12) 比阳性肿瘤 (582 +/- 212) 低得多，因此类似于 HPV18 阳性肿瘤。病毒拷贝数与肿瘤的临床分期呈负相关，并与组织学分化程度直接相关。然而，这些相关性主要归因于完整 E1/E2 区域的存在或不存在。 (C) 1994 年 Wiley-Liss 公司
The level of amplification (copy number/cell) of HPV16 and HPV18 viral genomes and its correlation with the presence of E1/E2 genes were analyzed in a sample of 42 HPV16- and 21 HPV18-positive cervical carcinomas of different clinical stages and histological types. The viral copy number/cell was assessed by dot-blot hybridization and the presence of E1/E2 genes by PCR and Southern blot. The copy number/cell was significantly lower in HPV18-positive than in HPV16-positive tumours (23 +/- 8 and 457 +/- 191 respectively). Nearly half of the HPV16s (43%) were distributed similarly to the HPV18s int he ranges of 50 or less copies, having its peak at the group of 1 to 10 copies, whereas the remaining HPV16s (57%) spread over the groups of 51 or more copies, with another peak at the group of 101 to 500. The E1/E2 region was absent in all tumours positive for HPV18 and present in 64% of those positive for HPV16. The HPV16 tumours negative for E1/E2 had a much lower viral copy number (17 +/- 12) than the positive ones (582 +/- 212), thus resembling HPV18-positive tumours. Viral copy number was negatively correlated with the clinical stage of the tumours and directly associated with the degree of histological differentiation. However, these correlations are primarily attributable to the presence or absence of an intact E1/E2 region. (C) 1994 Wiley-Liss, Inc.