SHORT-RANGE DNA LOOPING BY THE XENOPUS HMG-BOX TRANSCRIPTION FACTOR, XUBF
SHORT-RANGE DNA LOOPING BY THE XENOPUS HMG-BOX TRANSCRIPTION FACTOR, XUBF
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DOI：
10.1126/science.8178172
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发表时间：
1994-05-20
期刊：
影响因子：
56.9
通讯作者：
MOSS, T
中科院分区：
文献类型：
--
作者：
BAZETTJONES, DP;LEBLANC, B;MOSS, T
爪蟾UBF （xUBF）通过多个HMG-box结构域与DNA相互作用。当xUBF与核糖体启动子结合时，羧基端酸性尾和氨基端HMG-box相互作用。结合也导致负DNA超卷曲，形成盘状结构，增强。在增强区域内，一个xUBF二聚体产生一个低密度的蛋白质核心，DNA在其周围形成一个180碱基对的单圈，可能是通过同相弯曲。这种结构的增强提示了一种依赖于xubf的TATA盒结合蛋白复合体募集机制，而这两种因子之间没有直接的相互作用。
Xenopus UBF (xUBF) interacts with DNA by way of multiple HMG-box domains. When xUBF binds to the ribosomal promoter, the carboxyl-terminal acidic tail and amino-terminal HMG-box interact. Binding also leads to negative DNA supercoiling and the formation of a disk-like structure, the enhancesome. Within the enhancesome, an xUBF dimer makes a low-density protein core around which DNA is looped into a single 180-base pair turn, probably by in-phase bending. The enhancesome structure suggests a mechanism for xUBF-dependent recruitment of the TATA box-binding protein complex without direct interaction between the two factors.