Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease.
Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease.
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DOI：
10.1155/2022/7294775
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发表时间：
2022
影响因子：
2
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中科院分区：
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--
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大量研究致力于阐明内脏高敏感性的发病机制，以指导腹泻型肠易激综合征（IBS-D）的治疗。瞬时受体电位（TRP）通道及其在内脏伤害感受中的作用已得到广泛研究。我们研究了IBS-D患者结肠活检组织中TRP通道的表达情况及其与疾病严重程度的相关性。
从34例IBS-D患者和28名健康对照者（HCs）获取乙状结肠活检样本。IBS-D依据罗马IV标准进行诊断。通过问卷调查评估他们的临床参数。采用免疫组织化学染色法评估TRPV1、TRPV4、TRPA1、TRPM2和TRPM8的表达。
IBS-D患者结肠黏膜中TRPV1、TRPV4和TRPA1的表达水平显著高于健康对照者（p < 0.05），而IBS-D患者与健康对照者之间TRPM2和TRPM8的表达无明显差异。此外，结肠黏膜中TRPV1和TRPA1（而非TRPV4）的表达水平与疾病严重程度呈正相关（r分别为0.6303和0.4506，p < 0.05）。
与健康对照者相比，IBS-D患者结肠黏膜中TRPV1、TRPA1和TRPV4的表达增强，前两者与疾病严重程度相关。TRP通道可能是IBS-D诊断及疾病严重程度评估中有前景的生物标志物。
Lots of researches have endeavored to elucidate the pathogenetic mechanism of visceral hypersensitivity in order to guide the therapy of diarrhea predominant-irritable bowel syndrome (IBS-D). Transient receptor potential (TRP) channels and their role in visceral nociception have been vastly investigated. We investigated the expression of TRP channels in IBS-D colonic biopsies and its correlation with the severity of the disease. Sigmoid biopsies were obtained from 34 IBS-D patients and 28 healthy controls (HCs). IBS-D was diagnosed according to Rome IV criteria. Their clinical parameters were assessed through questionnaires. Expression of TRPV1, TRPV4, TRPA1, TRPM2, and TRPM8 was evaluated with immunohistology staining. Expression levels of TRPV1, TRPV4, and TRPA1 in the colonic mucosa of IBS-D patients were significantly higher than those in HCs (p < 0.05), while there was no obvious difference of TRPM2 and TRPM8 expression between IBS-D patients and HCs. In addition, the expression levels of TRPV1 and TRPA1, but TRPV4, in the colonic mucosa correlated positively with the severity of diseases (r = 0.6303 and 0.4506, respectively, p < 0.05). Expression of TRPV1, TRPA1, and TRPV4 in the colonic mucosa was enhanced in IBS-D patients compared with HCs with the former two correlated with the severity of the disease. TRP channels might be promising biomarkers in the diagnosis and estimate of the severity in IBS-D.
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