GLUCAGON IN PHYSIOLOGICAL CONCENTRATIONS STIMULATES BROWN FAT THERMOGENESIS INVIVO
GLUCAGON IN PHYSIOLOGICAL CONCENTRATIONS STIMULATES BROWN FAT THERMOGENESIS INVIVO
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DOI：
10.1152/ajpregu.1991.261.2.r501
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发表时间：
1991-08-01
影响因子：
--
通讯作者：
LEVINE, AS
中科院分区：
文献类型：
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作者：
BILLINGTON, CJ;BRIGGS, JE;LEVINE, AS
我们的目的是进一步表征胰高血糖素对棕色脂肪的刺激作用，并检验生理水平的高胰高血糖素血症会刺激棕色脂肪产热的假设。 在第一组实验中，胰高血糖素（1 mg/kg sc，每日两次）或溶剂对照在26 h内给药3次。 这种大剂量的胰高血糖素使GDP与棕色脂肪线粒体的结合增加。 此外，Scatchard分析表明胰高血糖素诱导的GDP结合位点数量增加，而没有证据表明结合位点亲和力发生改变。 在单次注射胰高血糖素（1 mg/kg）后2 h，棕色脂肪线粒体GDP结合率没有持续增加。 在第二组实验中，胰高血糖素通过恒定渗透微泵输注腹膜内给药。 胰高血糖素以150 μ g·kg-1·day-1的剂量给药5天，使GDP与棕色脂肪线粒体的结合显著增加，而胰高血糖素血清水平增加，但保持在通常的生理范围内。 通过恒定输注给予的较大剂量的胰高血糖素在7天内几乎消除了体重增加，同时显著增加了与棕色脂肪线粒体的核苷酸结合（GDP）。 胰高血糖素在产热调节中的重要作用。
Our aims were to further characterize the stimulatory effect of glucagon on brown fat and to test the hypothesis that physiological levels of hyperglucagonemia would stimulate brown fat thermogenesis. In the first set of experiments, glucagon (1 mg/kg sc twice daily) or vehicle control was administered three times in 26 h. This large dose of glucagon produced increases in GDP binding to brown fat mitochondria. In addition, Scatchard analysis indicated a glucagon-induced increase in number of GDP binding sites without evidence for alteration in binding site affinity. No consistent increase in brown fat mitochondrial GDP binding was produced 2 h after a single injection of glucagon (1 mg/kg). In the second set of experiments, glucagon was administered intraperitoneally by constant osmotic minipump infusion. Glucagon in a dose of 150-mu-g.kg-1.day-1 for 5 days produced significant increases in GDP binding to brown fat mitochondria, whereas glucagon serum levels were increased but stayed within the usual physiological range. A larger dose of glucagon administered by constant infusion virtually eliminated body weight gain over 7 days while significantly increasing nucleotide binding (GDP) to brown fat mitochondria. An important role for glucagon in thermogenic regulation is suggested.