Characterization of a new serotype g isolate of Aggregatibacter actinomycetemcomitans.
Characterization of a new serotype g isolate of Aggregatibacter actinomycetemcomitans.
复制标题
放线聚集杆菌新血清型 g 分离株的表征。
DOI：
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发表时间：
2010
影响因子：
3.7
通讯作者：
M. Hirasawa
中科院分区：
文献类型：
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作者：
K. Takada;M. Saito;O. Tsuzukibashi;Y. Kawashima;S. Ishida;M. Hirasawa
伴生放线杆菌通常是从口腔中分离出来的，在口腔中与活动性牙周炎有关。根据其表面碳水化合物抗原，该种可分为6个血清型(a-f)。然而，一些临床分离株不能归入这六个血清型。从1例牙周炎患者中分离出革兰氏阴性、兼性厌氧、过氧化氢酶阳性球菌，并通过遗传学、生化和血清学分析进行鉴定。表型鉴定结果表明，该菌株为伴生放线菌。免疫扩散试验表明，该分离株的血清型特异性多糖抗原与放线菌a~f血清型菌株相比不能分型。该菌株生物被膜形成能力强，但细胞毒活性低。从表面多糖中分离得到部分甲基化的糖醇乙酸酯，经气相色谱/质谱分析表明，含有2，4-二-O-甲基-鼠李糖和2，3，6-三-O-甲基葡萄糖，比例为1：1。该抗原的(1)H-和(13)C-核磁共振波谱表明，两个组分的糖基都具有α-异构体构型。推测该未分型菌株是一种新的伴生放线放线菌血清型，命名为g型。
Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a-f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram-negative, facultative anaerobic, catalase-positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype-specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4-di-O-methyl-rhamnose and 2,3,6-tri-O-methyl-glucose, with a 1 : 1 m ratio. The (1)H- and (13)C-nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had alpha-anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.