Complexity of RNA in eggs of Drosophila melanogaster and Musca domestica.
Complexity of RNA in eggs of Drosophila melanogaster and Musca domestica.
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果蝇和家蝇卵中 RNA 的复杂性。
DOI：
10.1093/genetics/95.1.81
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Hough-Evans,BR;Jacobs-Lorena,M;Cummings,MR;Britten,RJ;Davidson,EH
对储存在两种双翅目苍蝇卵中的RNA的序列复杂性进行了比较测量，家蝇和黑腹果蝇的基因组大小约为果蝇基因组的5倍，单拷贝序列约为果蝇基因组的3.6倍。如前所述，在蝇类中，重复和单拷贝序列的点缀是短周期形式，而在果蝇中是长周期形式。通过过量的RNA与放射性标记的单拷贝DNA杂交来确定鸡蛋RNA的复杂性。复杂性表示为RNA中所代表的各种单拷贝序列的长度(以核苷酸为单位)。蝇卵的RNA复杂性约为2.4×107个核苷酸，果蝇卵的RNA复杂性约为1.2×107个核苷酸。蝇卵的RNA的复杂性类似于或略低于其他卵的RNA，例如非洲爪哇和海胆的RNA。与以前测得的所有卵RNA相比，果蝇卵RNA的序列复杂性较低。
Comparative measurements are presented of the sequence complexity of the RNA stored in the eggs of two dipteran flies,Musca domesticaandDrosophila melanogaster.The genome of Musca is about five times the size of the Drosophila genome and contains about 3.6 times as much single-copy sequence. As shown earlier, the interspersion of repetitive and single-copy sequence is of the short-period form in Musca, and is of the long-period form in Drosophila. The egg RNA complexities were determined by hybridization of excess RNA with radioactively labeled single-copy DNA. Complexity is expressed as the length (in nucleotides) of diverse single-copy sequence representedinthe RNA. The complexity of the RNA of the Musca egg is about 2.4 x 107 nucleotides, and that of the Drosophila egg is about 1.2 x 107 nucleotides. The RNA of the Musca egg is similartoor very slightly lower in complexity than that of other egg RNAs,e.g.,those of Xenopus and sea urchin. Comparedtoall previously measured egg RNAs, Drosophila egg RNA is low in sequence complexity.