Okadaic acid inhibits activation of K-Cl cotransport in red blood cells containing hemoglobins S and C.
Okadaic acid inhibits activation of K-Cl cotransport in red blood cells containing hemoglobins S and C.
复制标题
冈田酸抑制含有血红蛋白 S 和 C 的红细胞中 K-Cl 共转运的激活。
DOI：
10.1152/ajpcell.1991.261.4.c591
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Parker,JC
中科院分区：
文献类型：
--
作者：
Orringer,EP;Brockenbrough,JS;Whitney,JA;Glosson,PS;Parker,JC
含有血红蛋白 S 和 C 的红细胞对通过渗透膨胀和酸化激活 K-Cl 共转运的敏感性被冈田酸（一种特定的蛋白磷酸酶抑制剂）降低。大田酸的剂量反应曲线表明其作用于 1 型蛋白磷酸酶。冈田酸先前已被证明可以抑制兔子、正常人和狗的红细胞中肿胀诱导的 K-Cl 共转运激活。目前的工作证实了大田酸减弱细胞肿胀对 K-Cl 共转运的刺激的观察结果。新信息是，冈田酸可降低血红蛋白 S 和 C 对 K-Cl 共转运的体积和 pH 敏感性的影响。因此，细胞体积、pH 和突变血红蛋白的影响可能都是通过影响 K-Cl 磷酸化状态的常见机制介导的。协同转运蛋白本身或调节其功能的蛋白质的协同转运蛋白。
The sensitivity of red blood cells containing hemoglobins S and C to activation of K-Cl cotransport by osmotic swelling and acidification was reduced by okadaic acid, a specific protein phosphatase inhibitor. The dose-response curve for okadaic acid suggests its action is on a type 1 protein phosphatase. Okadaic acid has been previously shown to inhibit swelling-induced activation of K-Cl cotransport in red blood cells from rabbits, normal humans, and dogs. The present work confirms the observation that okadaic acid blunts the stimulation of K-Cl cotransport by cell swelling. The new information is that okadaic acid reduces the effects of hemoglobins S and C on the volume and pH sensitivity of K-Cl cotransport. Thus the influences of cell volume, pH, and mutant hemoglobins may all be mediated via a common mechanisms that affects the phosphorylation state, either of the K-Cl. cotransporter itself or of a protein that regulates its function.