Osteoblastic cellular responses to aluminosilicate nanotubes, imogolite using Saos-2 and MC3T3-E1 cells
Osteoblastic cellular responses to aluminosilicate nanotubes, imogolite using Saos-2 and MC3T3-E1 cells
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DOI：
10.2109/jcersj2.118.516
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发表时间：
2010-06
影响因子：
1.1
通讯作者：
K. Ishikawa;S. Abe;Y. Yawaka;Masaya Suzuki;F. Watari
中科院分区：
文献类型：
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作者：
K. Ishikawa;S. Abe;Y. Yawaka;Masaya Suzuki;F. Watari
伊毛缟石是一种天然存在的水合铝硅酸盐粘土矿物。它具有类似于单壁碳纳米管的纳米管状结构。伊毛缟石是土壤学领域感兴趣的一个主题，但迄今为止，这种物质还没有生物应用。在这项研究中，我们研究了成骨细胞样细胞对伊毛缟石支架的细胞反应。为了揭示形态、增殖和成骨细胞功能矿化，我们在支架上培养了人成骨细胞样细胞（Saos-2）和小鼠成骨细胞样细胞（MC3T3-E1）。伊毛缟石支架的表面特性根据培养皿上伊毛缟石的量而发生巨大变化。随着伊缟石浓度的增加，支架的表面形貌从随机取向的岛状结构转变为同向排列的自组织纤维结构，最终形成多层随机取向的全覆盖。与传统培养皿（对照）中的外观相比，两种类型的成骨细胞都广泛分布在伊毛缟石支架上。两种类型的成骨细胞的增殖显示出潜在有用的趋势。在细胞功能方面，伊毛缟石支架上两种类型的成骨细胞的矿化程度均高于对照组。 Saos-2 和 MC3T3-E1 细胞除了增强的成骨细胞矿化外，还表现出良好的生物相容性。因此，伊毛缟石有望作为骨组织工程的支架材料。
Imogolite is a naturally occurring hydrous aluminosilicate clay mineral. It has nano-tubular structure similar to that of single-walled carbon nanotubes. Imogolite is a subject of interest in the pedological field, but to date there has been no bioapplications of this substance. In this study, we investigated the cellular response of osteoblast-like cells to imogolite scaffolds. To reveal the morphology, proliferation and osteoblastic functional mineralization, we cultured human osteoblast-like cells (Saos-2) and mouse osteoblast-like cells (MC3T3-E1) on the scaffolds. The surface characteristics of the imogolite scaffold were drastically changed depending on the amount of imogolite on the dish. With the increase of imogolite concentration, the surface morphology of scaffolds changed from an island-like shape in random orientation to a self-organized fiber texture aligned in the same direction, and finally full coverage in a random orientation with plural layers. Both types of osteoblast were widely spread on the imogolite scaffolds compared to their appearance in a conventional culture dish (control). The proliferation of the two types of osteoblast showed potentially useful trends. In cellular function, there was greater mineralization of both types of osteoblast on imogolite scaffolds than that of the control. Saos-2 and MC3T3-E1 cells showed good biocompatibility in addition to the enhanced osteoblastic mineralization. Therefore, imogolite is expected to be useful as a scaffold material for bone tissue engineering.