THE NUCLEAR MATRIX - 3-DIMENSIONAL ARCHITECTURE AND PROTEIN-COMPOSITION
THE NUCLEAR MATRIX - 3-DIMENSIONAL ARCHITECTURE AND PROTEIN-COMPOSITION
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DOI：
10.1016/0092-8674(82)90446-9
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
CAPCO, DG;WAN, KM;PENMAN, S
细胞核的结构细丝网络在仍与细胞骨架相连的情况下制备完成。这个相对温和的过程去除了大约98%的DNA和至少86%的组蛋白。该基质由连接到细胞骨架框架的外核层以及内丝所包围。这些细丝直径在3-22 nm之间，并以三维网状网络组织，核仁在其中网状排列。用双洗涤剂将核基质从细胞骨架中分离出来，然后用核酸酶和高盐将其分成染色质部分和基质部分。双向凝胶电泳法显示，细胞骨架、染色质和核基质中的蛋白质差异很大。在所有组分中发现的一种主要蛋白质与肌动蛋白共聚焦。波形蛋白在很大程度上与核基质有关，可能是作为细丝外部的日冕。
The structural filament network of the nucleus is prepared while still connected to the cytoskeleton. The relatively gentle procedure removes about 98% of the DNA and at least 86% of the histones. The matrix is bounded by an outer nuclear lamina connected to the cytoskeletal framework, as well as the inner filaments. The filaments range in diameter from 3-22 nm, and are organized in a 3-dimensional anastomosing network in which nucleoli are enmeshed. The nuclear matrix is separated from the cytoskeletal framework by a double detergent and then partitioned into a chromatin fraction and a matrix fraction by nuclease and high salt. Two-dimensional gel electrophoresis shows that the proteins of the cytoskeleton, chromatin and nucelar matrix are very different. A major protein found in all fractions cofocuses with actin. Vimentin is largely associated with the nuclear matrix, probably as a corona external of filaments.