Human c-myc and N-ras expression during induction of HL-60 cellular differentiation.
Human c-myc and N-ras expression during induction of HL-60 cellular differentiation.
复制标题
HL-60 细胞分化诱导过程中人 c-myc 和 N-ras 的表达。
DOI：
10.1016/0006-291x(85)90284-0
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Kufe,D
中科院分区：
文献类型：
--
作者：
Watanabe,T;Sariban,E;Mitchell,T;Kufe,D
人类 HL-60 早幼粒细胞白血病细胞的基因组包含扩增的 c-myc 序列和转化的 N-ras 癌基因。本研究监测了用二甲亚砜、六亚甲基双乙酰胺、12-0-十四烷酰佛波醇-13-乙酸酯和 1,25 二羟基维生素 D3 诱导骨髓和单核细胞分化过程中 HL-60 细胞中 c-myc 和 N-ras 的表达。结果表明，HL-60 分化的诱导与 c-myc RNA 的减少有关，而对 N-ras 基因的表达几乎没有影响。尽管c-myc表达的减少作为HL-60分化诱导的早期事件发生，但c-myc转录本的减少率随着增殖停止而变化。因此，成熟表型的出现和增殖能力的丧失与c-myc RNA的下降有关，而这些事件似乎是在N-ras表达没有显着改变的情况下发生的。
The genome of the human HL-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming N-ras oncogene. The present study has monitored c-myc and N-ras expression in HL-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethyline bisacetamide, 12-0-tetradcanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin D3. The results demonstrate that induction of HL-60 differentiation is associated with decreases in c-myc RNA, while there is little if any effect on expression of the N-ras gene. Although the diminution in c-myc expression occurred as an early event in the induction of HL-60 differentiation, the rate of decrease in c-myc transcripts varied with respect to cessation of proliferation. Thus, the appearance of the mature phenotype and loss of proliferative capacity are associated with declines in c-myc RNA, while these events appear to occur in the absence of significant alterations in N-ras expression.