TRANSFORMATION OF RAT FIBROBLASTS BY FSV RAPIDLY INCREASES GLUCOSE TRANSPORTER GENE-TRANSCRIPTION
TRANSFORMATION OF RAT FIBROBLASTS BY FSV RAPIDLY INCREASES GLUCOSE TRANSPORTER GENE-TRANSCRIPTION
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DOI：
10.1126/science.3029870
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发表时间：
1987-03-20
期刊：
影响因子：
56.9
通讯作者：
ROSEN, OM
中科院分区：
文献类型：
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作者：
BIRNBAUM, MJ;HASPEL, HC;ROSEN, OM
葡萄糖转运的升高是大多数病毒诱导的肿瘤常见的改变。研究了用野生型或温度敏感的藤波肉瘤病毒（FSV）转化的大鼠成纤维细胞，以确定增加运输的机制。转化后总细胞葡萄糖转运蛋白增加5 - 10倍，同时转运蛋白信使RNA水平也有类似的增加。反过来，在此之前，葡萄糖转运蛋白基因转录速率的绝对增加后30分钟内的温度敏感的FSV转化的细胞转移到允许的温度。转化成纤维细胞中的转运蛋白信使RNA水平高于维持在非允许温度下的增殖细胞中发现的水平。通过转化激活转运蛋白基因转录代表了肿瘤发生对编码功能明确的蛋白质的基因表达的最早已知影响之一。
Elevation of glucose transport is an alteration common to most virally induced tumors. Rat fibroblasts transformed with wild-type or a temperature-sensitive Fujinami sarcoma virus (FSV) were studied in order to determine the mechanisms underlying the increased transport. Five- to tenfold increases in total cellular glucose transporter protein in response to transformation were accompanied by similar increases in transporter messenger RNA levels. This, in turn, was preceded by an absolute increase in the rate of glucose transporter gene transcription within 30 minutes after shift of the temperature-sensitive FSV-transformed cells to the permissive temperature. The transporter messenger RNA levels in transformed fibroblasts were higher than those found in proliferating cells maintained at the nonpermissive temperature. The activation of transporter gene transcription by transformation represents one of the earliest known effects of oncogenesis on the expression of a gene encoding a protein of well-defined function.