P53 MODULATION OF TFIIH-ASSOCIATED NUCLEOTIDE EXCISION-REPAIR ACTIVITY
P53 MODULATION OF TFIIH-ASSOCIATED NUCLEOTIDE EXCISION-REPAIR ACTIVITY
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DOI：
10.1038/ng0695-188
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发表时间：
1995-06-01
期刊：
影响因子：
30.8
通讯作者：
HARRIS, CC
中科院分区：
文献类型：
--
作者：
WANG, XW;YEH, H;HARRIS, CC
p53具有多效性功能，包括控制基因组的可塑性和完整性。在这里，我们报告说，p53可以结合到几个转录因子IIH相关的因素，包括转录修复因子，XPD（Rad 3）和XPB，以及CSB参与链特异性DNA修复，通过其C-末端结构域。我们还发现，野生型，但不是Arg 273 His突变体p53抑制XPD（Rad 3）和XPB DNA解旋酶活性。此外，在Li-Fraumeni综合征细胞（杂合子p53突变体）中，UV诱导的二聚体的修复比正常人细胞慢。我们的研究结果表明，p53可能在调节核苷酸切除修复途径中发挥直接作用。
p53 has pleiotropic functions including control of genomic plasticity and integrity. Here we report that p53 can bind to several transcription factor IIH-associated factors, including transcription-repair factors, XPD (Rad3) and XPB, as well as CSB involved in strand-specific DNA repair, via its C-terminal domain. We also found that wild-type, but not Arg273His mutant p53 inhibits XPD (Rad3) and XPB DNA helicase activities. Moreover, repair of UV-induced dimers is slower in Li-Fraumeni syndrome cells (heterozygote p53 mutant) than in normal human cells. Our findings indicate that p53 may play a direct role in modulating nucleotide excision repair pathways.