Intracisternal basic fibroblast growth factor enhances functional recovery and up-regulates the expression of a molecular marker of neuronal sprouting following focal cerebral infarction
Intracisternal basic fibroblast growth factor enhances functional recovery and up-regulates the expression of a molecular marker of neuronal sprouting following focal cerebral infarction
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DOI：
10.1073/pnas.94.15.8179
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发表时间：
1997-07-22
影响因子：
11.1
通讯作者：
Finklestein, SP
中科院分区：
文献类型：
--
作者：
Kawamata, T;Dietrich, WD;Finklestein, SP
成年大鼠单侧大脑中动脉闭塞导致的局灶性脑梗死（中风）会导致对侧肢体感觉运动功能的缺陷，并随时间推移而部分恢复。我们发现每两周脑池内注射碱性成纤维细胞生长因子（bFGF; 0.5 μ g/注射），一种有效的神经营养多肽，在中风后的第一个月内显著增强对侧肢体感觉运动功能的恢复，而没有明显的不良副作用，对生长相关蛋白43（GAP-43）进行免疫染色，结果显示，bFGF治疗后，脑梗死对侧完整感觉运动皮层中GAP-43免疫反应性选择性增加。这些结果表明，bFGF治疗可促进卒中后功能恢复，其机制可能包括刺激完整脑中的神经元发芽。
Focal cerebral infarction (stroke) due to unilateral occlusion of the middle cerebral artery in mature rats produces deficits in sensorimotor function of the contralateral limbs that recover partially over time. We found that biweekly intracisternal injection of basic fibroblast growth factor (bFGF; 0.5 mu g/injection), a potent neurotrophic polypeptide, markedly enhanced recovery of sensorimotor function of the contralateral limbs during the first month after stroke without apparent adverse side effects, Immunostaining for growth-associated protein 43 (GAP-43), a molecular marker of axonal sprouting, showed a selective increase in GAP-43 immunoreactivity in the intact sensorimotor cortex contralateral to cerebral infarcts following bFGF treatment, These results show that bFGF treatment can enhance functional recovery after stroke, and that the mechanism may include stimulation of neuronal sprouting in the intact brain.